AtCSLD3 and GhCSLD3 mediate root growth and cell elongation downstream of the ethylene response pathway in Arabidopsis.
AtCSLD3 and GhCSLD3 mediate root growth and cell elongation downstream of the ethylene response pathway in Arabidopsis.
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AtCSLD3 和 GhCSLD3 介导拟南芥乙烯响应途径下游的根生长和细胞伸长
DOI：
10.1093/jxb/erx470
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发表时间：
2018-02-23
影响因子：
6.9
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Hu H;Zhang R;Dong S;Li Y;Fan C;Wang Y;Xia T;Chen P;Wang L;Feng S;Persson S;Peng L
CSLD3过表达通过增加细胞伸长来促进根和下胚轴的生长；csld3介导的下胚轴伸长与乙烯信号无关。CSLD3是纤维素合酶样D家族的一个基因，影响根毛伸长，但其与乙烯信号和磷酸盐饥饿的相互作用尚不清楚。在这里，我们的目的是了解CSLD3在拟南芥植物生长中乙烯信号传导和磷酸盐饥饿途径中的作用。因此，我们对csld3-1突变体、csld3过表达系和乙烯反应突变体（如组成型乙烯反应突变体i-ctr1）进行了比较分析。我们发现CSLD3过表达通过增加细胞伸长来促进根和下胚轴的生长，并且根的生长对乙烯处理（1µM ACC）高度敏感，特别是在磷酸盐饥饿下。然而，csld3介导的下胚轴伸长独立于乙烯信号通路发生。值得注意的是，乙烯和磷酸盐饥饿对根毛和根伸长的诱导作用在csld3-1突变体中被完全消除。此外，i-ctr1 CSLD3 -1双突变体与CSLD3 -1亲本一样无毛，证实了CSLD3在根生长过程中作用于乙烯信号通路的下游。此外，CSLD3水平与纤维素水平正相关，表明CSLD3在纤维素合成中起作用，这可能解释了观察到的生长效应。我们的研究结果确定了CSLD3在根毛生长、细胞伸长和细胞壁生物合成过程中如何在乙烯信号传导和磷酸盐饥饿途径中起作用。
CSLD3 overexpression enhances root and hypocotyl growth by increasing cell elongation; CSLD3-mediated hypocotyl elongation is independent of ethylene signaling. CSLD3, a gene of the cellulose synthase-like D family, affects root hair elongation, but its interactions with ethylene signaling and phosphate-starvation are poorly understood. Here, we aim to understand the role of CSLD3 in the context of the ethylene signaling and phosphate starvation pathways in Arabidopsis plant growth. Therefore, we performed a comparative analysis of the csld3-1 mutant, CSLD3-overexpressing lines, and ethylene-response mutants, such as the constitutive ethylene-response mutant i-ctr1. We found that CSLD3 overexpression enhanced root and hypocotyl growth by increasing cell elongation, and that the root growth was highly sensitive to ethylene treatment (1 µM ACC), in particular under phosphate starvation. However, the CSLD3-mediated hypocotyl elongation occurred independently of the ethylene signaling pathway. Notably, the typical induction of root hair and root elongation by ethylene and phosphate-starvation was completely abolished in the csld3-1 mutant. Furthermore, i-ctr1 csld3-1 double-mutants were hairless like the csld3-1 parent, confirming that CSLD3 acts downstream of the ethylene signaling pathway during root growth. Moreover, the CSLD3 levels positively correlated with cellulose levels, indicating a role of CSLD3 in cellulose synthesis, which may explain the observed growth effects. Our results establish how CSLD3 works in the context of the ethylene signaling and phosphate-starvation pathways during root hair growth, cell elongation, and cell wall biosynthesis.
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影响因子：
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作者：
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