Nano-pulse stimulation (NPS) ablate tumors and inhibit lung metastasis on both canine spontaneous osteosarcoma and murine transplanted hepatocellular carcinoma with high metastatic potential.
Nano-pulse stimulation (NPS) ablate tumors and inhibit lung metastasis on both canine spontaneous osteosarcoma and murine transplanted hepatocellular carcinoma with high metastatic potential.
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纳米脉冲刺激（NPS）可消融犬自发性骨肉瘤和具有高转移潜力的小鼠移植性肝细胞癌的肿瘤并抑制肺转移
DOI：
10.18632/oncotarget.17178
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发表时间：
2017-07-04
期刊：
影响因子：
--
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Chen X;Chen Y;Jiang J;Wu L;Yin S;Miao X;Swanson RJ;Zheng S
背景纳秒脉冲电场(NsPEF)，又称纳米脉冲刺激(NPS)，具有刺激肿瘤细胞免疫应答的潜能。本研究旨在探讨其在晚期肿瘤肺转移中的局部和全身抗肿瘤作用。方法将12只自发性骨肉瘤犬和12只人肝癌移植瘤裸鼠随机分为3组，分别给予NPS治疗、手术治疗和不治疗对照。采用40千伏/厘米的穿透电极、500个脉冲、频率为1赫兹的纳秒脉冲电场。术后随访生存时间、肿瘤体积、血清碱性磷酸酶(ALP)、关节囊损伤及肺转移情况。并与对照组进行疗效比较。结果与对照组相比，纳秒脉冲电场能显著缩小原发肿瘤体积，延长生存期(P<；0.05)。对血清碱性磷酸酶和肺转移的抑制作用也未见关节畸形和热损伤。结论局部应用纳秒脉冲电场是一种新的非热消融方法。它可以消融原发肿瘤，减少肺转移，是晚期肿瘤的姑息性治疗。
Background Nanosecond pulsed electric field (nsPEF), which is also termed as nano-pulse stimulation (NPS), has the potential of stimulating immune responses toward cancer cells. The current study investigates its local and systemic antitumor efficacy in vivo in late stage tumors with lung metastasis. Method The 12 canines with spontaneous osteosarcomas and 12 nude mice transplanted with human hepatocellular carcinoma were divided randomly and were given NPS treatment, surgery or no treatment control. Nanosecond pulsed electric field was delivered with puncture electrodes at 40 kV/cm with 500 pulses at 1 Hz. The survival time, tumor volume, serum alkaline phosphatase (ALP), joint capsule damage and lung metastasis were followed up. The efficacy was compared with control. Results Nanosecond pulsed electric field reduced primary tumor volume and extended the survival significantly compared to the control group (P<0.05). Inhibition of serum alkaline phosphatase and lung metastasis without joint deformity or thermal damage were also observed. Conclusion Locally applied nanosecond pulsed electric field is a novel non-thermal ablation method. It can ablate the primary tumor and decrease lung metastasis as a palliative therapy for late stage tumor.
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