Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans.
Single-cell transcriptional analysis of taste sensory neuron pair in Caenorhabditis elegans.
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DOI：
10.1093/nar/gkp868
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发表时间：
2010-01
影响因子：
14.9
通讯作者：
Iino Y
中科院分区：
文献类型：
--
作者：
Takayama J;Faumont S;Kunitomo H;Lockery SR;Iino Y
神经系统是由各种各样的神经元组成。对每个神经元的转录谱的描述将产生关于定义形态或功能特征的分子机制的大量信息。在这里，我们表明，从单个神经元的RNA分离是可行的，通过使用优化的mRNA标记方法。该方法通过RNA和在细胞中特异性表达的表位标记的poly（A）结合蛋白的复合物的共免疫沉淀来提取靶细胞中的转录物。利用这种方法和全基因组微阵列，我们比较了两个功能不同的神经元在主C。Elegans味觉神经元ASE类。13个已知亚型特异性基因中有8个被成功检测到。此外，我们确定了9个新的基因，包括受体鸟苷酸环化酶，分泌蛋白，TRPC通道和线虫中保守的未表征基因，这表明这两个神经元与以前认为的有很大不同。这些新基因的表达是由先前已知的亚型分化调控网络控制的。我们还描述了独特的基序组织内的个体基因组分类的表达模式在ASE。我们的研究铺平了道路，以完整的目录的表达谱的个人C。线虫神经元
The nervous system is composed of a wide variety of neurons. A description of the transcriptional profiles of each neuron would yield enormous information about the molecular mechanisms that define morphological or functional characteristics. Here we show that RNA isolation from single neurons is feasible by using an optimized mRNA tagging method. This method extracts transcripts in the target cells by co-immunoprecipitation of the complexes of RNA and epitope-tagged poly(A) binding protein expressed specifically in the cells. With this method and genome-wide microarray, we compared the transcriptional profiles of two functionally different neurons in the main C. elegans gustatory neuron class ASE. Eight of the 13 known subtype-specific genes were successfully detected. Additionally, we identified nine novel genes including a receptor guanylyl cyclase, secreted proteins, a TRPC channel and uncharacterized genes conserved among nematodes, suggesting the two neurons are substantially different than previously thought. The expression of these novel genes was controlled by the previously known regulatory network for subtype differentiation. We also describe unique motif organization within individual gene groups classified by the expression patterns in ASE. Our study paves the way to the complete catalog of the expression profiles of individual C. elegans neurons.
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影响因子：
9.2
作者：
Colosimo, ME;Brown, A;Sengupta, P
通讯作者：
Sengupta, P
影响因子：
64.5
作者：
Malnic, B;Hirono, J;Buck, LB
通讯作者：
Buck, LB
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.6
作者：
Etchberger, John F.;Flowers, Eileen B.;Hobert, Oliver
通讯作者：
Hobert, Oliver
影响因子：
12.3
作者：
Kunitomo, H;Uesugi, H;Iino, Y
通讯作者：
Iino, Y