A brain-wide analysis maps structural evolution to distinct anatomical module.
A brain-wide analysis maps structural evolution to distinct anatomical module.
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DOI：
10.7554/elife.80777
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发表时间：
2023-07-27
期刊：
影响因子：
7.7
通讯作者：
Duboue ER
中科院分区：
文献类型：
--
作者：
Kozol RA;Conith AJ;Yuiska A;Cree-Newman A;Tolentino B;Benesh K;Paz A;Lloyd E;Kowalko JE;Keene AC;Albertson C;Duboue ER
脊椎动物大脑在拓扑上高度保守，但对各个大脑区域之间的神经解剖学差异知之甚少。假设区域水平的神经解剖学变异可以提供功能扩展，建立在基本功能所需的祖先解剖结构的基础上。传统上，用于研究进化的动物模型缺乏广泛用于斑马鱼和小鼠的详细解剖分析工具，这给精细尺度的大脑进化研究带来了障碍。在这项研究中，我们试图使用由不同的表面和洞穴形态组成的单一鱼类来研究大脑解剖结构的进化，这允许对整个大脑的区域体积和形状进行功能基因测试。我们为墨西哥盲穴鱼 Astyanax mexicanus 生成了高分辨率的大脑图谱，并将该图谱与自动化计算工具结合起来，以直接评估所有种群的大脑区域形状和体积的变异性。我们测量了大脑中每个粗略定义的神经解剖区域的体积和形状，并评估了地表鱼、洞穴鱼和地表×洞穴F2杂交种的解剖区域之间的相关性，这些鱼的表型跨越了地表到洞穴的范围。我们发现大脑的背侧区域收缩，而腹侧区域扩张，F2 混合数据为沿背腹轴的发育限制提供了支持。此外，这些解剖变异中的背腹关系在体积和形状上表现出相似的模式，表明这两个参数捕获的解剖进化可能是由相似的发育机制驱动的。总之，这些数据表明，A. mexicanus 是一个功能强大的系统，可以在功能上确定大脑进化的基本原理，并将允许测试基因如何影响早期模式事件以驱动全脑解剖进化。
The vertebrate brain is highly conserved topologically, but less is known about neuroanatomical variation between individual brain regions. Neuroanatomical variation at the regional level is hypothesized to provide functional expansion, building upon ancestral anatomy needed for basic functions. Classically, animal models used to study evolution have lacked tools for detailed anatomical analysis that are widely used in zebrafish and mice, presenting a barrier to studying brain evolution at fine scales. In this study, we sought to investigate the evolution of brain anatomy using a single species of fish consisting of divergent surface and cave morphs, that permits functional genetic testing of regional volume and shape across the entire brain. We generated a high-resolution brain atlas for the blind Mexican cavefish Astyanax mexicanus and coupled the atlas with automated computational tools to directly assess variability in brain region shape and volume across all populations. We measured the volume and shape of every grossly defined neuroanatomical region of the brain and assessed correlations between anatomical regions in surface fish, cavefish, and surface × cave F2 hybrids, whose phenotypes span the range of surface to cave. We find that dorsal regions of the brain are contracted, while ventral regions have expanded, with F2 hybrid data providing support for developmental constraint along the dorsal-ventral axis. Furthermore, these dorsal-ventral relationships in anatomical variation show similar patterns for both volume and shape, suggesting that the anatomical evolution captured by these two parameters could be driven by similar developmental mechanisms. Together, these data demonstrate that A. mexicanus is a powerful system for functionally determining basic principles of brain evolution and will permit testing how genes influence early patterning events to drive brain-wide anatomical evolution.
影响因子：
16.6
作者：
Gomez-Robles, Aida;Hopkins, William D.;Sherwood, Chet C.
通讯作者：
Sherwood, Chet C.
影响因子：
3.8
作者：
Earl, Brian
通讯作者：
Earl, Brian