Immunocytochemical Localization of Cathepsins B and G in Odontoclasts of Human Deciduous Teeth
Immunocytochemical Localization of Cathepsins B and G in Odontoclasts of Human Deciduous Teeth
复制标题
人乳牙破牙细胞中组织蛋白酶 B 和 G 的免疫细胞化学定位
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
E. Ueno
中科院分区：
文献类型：
--
作者：
T. Sasaki;E. Ueno
为了阐明破牙细胞在生理性牙根吸收过程中吸收B乳牙的机制，半胱氨酸蛋白酶如组织蛋白酶B和G在超微结构水平上免疫细胞化学定位于破牙细胞中。用甲醛和戊二醛的混合物固定正在进行牙根吸收的人乳牙，并进行这些酶的免疫细胞化学检测。使用抗人组织蛋白酶B或G的绵羊抗血清作为一抗。在破牙细胞中，抗组织蛋白酶B和G的特异性免疫金标记清楚地定位于溶酶体和各种大小的苍白空泡中，以及破牙细胞皱褶边界的细胞外管的一部分中。在任一抗体的存在下，细胞质基质、线粒体和细胞核被免疫金颗粒最低限度地标记。这些蛋白水解酶在破牙细胞中的存在表明，在破牙牙根吸收过程中，这些酶通过乳牙胶原蛋白和其他非胶原基质蛋白的细胞内/细胞外降解参与吸收陷窝的形成。
For clarification of the mechanisms by which odontoclasts resorb deciduous teeth during physiological root resorption, cysteine-proteinases such as cathepsins B and G were immunocytochemically localized in odontoclasts at the ultrastructural level. Extracted human deciduous teeth undergoing root resorption were fixed with a mixture of formaldehyde and glutaraldehyde and processed for immunocytochemical detection of these enzymes. Sheep antisera, raised against either human cathepsin B or G, were used as primary antibodies. In odontoclasts, specific immunogold labeling of both anti-cathepsin B and G was clearly localized in lysosomes and pale vacuoles of various sizes, and in a portion of the extracellular canals of odontoclastic ruffled borders. In the presence of either antibody, the cytoplasmic matrix, mitochondria, and nuclei were minimally labeled by immunogold particles. The presence of these proteolytic enzymes in odontoclasts suggests that, during odontoclastic root resorption, these enzymes are involved in the formation of resorption lacunae by means of intra/extracellular degradation of collagen and other non-collagenous matrix proteins of deciduous teeth.
分离的破骨细胞中的组织蛋白酶 B 和 L 活性。
DOI：
10.1016/0006-291x(91)91334-9
发表时间：
1991
影响因子：
3.1
作者：
Rifkin,BR;Vernillo,AT;Kleckner,AP;Auszmann,JM;Rosenberg,LR;Zimmerman,M
通讯作者：
Zimmerman,M