Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum.
Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum.
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DOI：
10.3389/fphys.2022.1024136
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
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昆虫几丁质酶在蜕皮过程中对外骨骼中几丁质的消化起着至关重要的作用。然而，与环境胁迫反应相关的昆虫几丁质酶的研究非常有限。本研究首次对非生物胁迫下豌豆几丁质酶相关基因的表达进行了分析。在此，我们鉴定了5个几丁质酶样蛋白(ApIDGF、ApCht3、ApCht7、ApCht10和ApENGase)，并将它们聚为5个组(组II、组III、组V、组Ⅹ和组5)。发育表达分析表明，5个豌豆根结线虫几丁质酶相关基因在整个发育阶段均有表达，但表达模式不同。在温度、杀虫剂和20-羟基蜕皮酮(20E)等非生物胁迫下，五种几丁质酶基因在豌豆根结线虫中均有差异表达。结果表明，吡虫啉等杀虫剂下调了这5个CHT相关基因的表达。对豌豆曲霉几丁质酶的温度胁迫分析表明，ApCht7在10℃时有较高的表达，表明其在低温下对豌豆蚜虫具有重要作用。相反，ApCht10在高温胁迫下更具活性，在30℃时显著上调。此外，20E增强了ApCht3和ApCht10在豌豆中的表达，但降低了ApCht7的表达。这些发现为研究这些基因在非生物胁迫下的作用提供了基本的信息和见解，促进了我们对多重胁迫下豌豆蚜虫管理的认识。
Insect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum under abiotic stresses. Here, we identified five chitinase-like proteins (ApIDGF, ApCht3, ApCht7, ApCht10 and ApENGase), and clustered them into five groups (group II, III, V, Ⅹ, and ENGase). Developmental expression analysis revealed that the five A. pisum chitinase-related genes were expressed at whole developmental stages with different relative expression patterns. When aphids were exposed to various abiotic stresses including temperature, insecticide and the stress 20-hydroxyecdysone (20E), all five chitinase genes were differentially expressed in A. pisum. The results showed that insecticide such as imidacloprid down-regulated the expression of these five Cht-related genes. Analysis of temperature stress of A. pisum chitinase suggested that ApCht7 expression was high at 10°C, which demonstrates its important role in pea aphids under low temperature. Conversely, ApCht10 was more active under high temperature stress, as it was significantly up-regulated at 30°C. Besides, 20E enhanced ApCht3 and ApCht10 expression in A. pisum, but reduced ApCht7 expression. These findings provide basic information and insights for the study of the role of these genes under abiotic stress, which advances our knowledge in the management of pea aphids under multiple stresses.
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