Molecular Variation and Genomic Function of Citrus Vein Enation Virus.
Molecular Variation and Genomic Function of Citrus Vein Enation Virus.
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柑橘叶脉病毒的分子变异和基因组功能
DOI：
10.3390/ijms24010412
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发表时间：
2022-12-27
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
本研究通过sRNA高通量测序和传统测序方法，鉴定了一株新的柑橘静脉生成病毒（CVEV）分离株（命名为CVEV- dt1）。基于所有已知CVEV分离株全基因组序列的系统发育分析表明，CVEV- dt1与中国其他分离株在一个进化分支上。分子变异分析表明，CVEV全长序列的单核苷酸变异小于8%，突变（60.55%）多于突变（39.43%），表明CVEV群体具有遗传同质性。此外，非同义核苷酸突变主要发生在ORF1和ORF2。基于cevv - dt1对所有编码ORF的失序分析，通过亚细胞定位和光漂白实验，我们发现cevv - dt1外壳蛋白（CP）形成球形颗粒，主要分布在细胞核内，部分分布在细胞质中，具有液体性质。此外，我们还证实了CVEV P0蛋白具有较弱的转录后rna沉默抑制活性，并可能在烟草植物中引起强烈的过敏反应（hypersensitive response， HR）。总的来说，据我们所知，我们的研究首次分析了所有报道的CVEV分离株的基因组变异，并揭示了CVEV- dt1编码蛋白的功能。
In this study, we identified a new citrus vein enation virus (CVEV) isolate (named CVEV-DT1) through sRNA high-throughput sequencing and traditional sequencing. Phylogenetic analysis based on whole genome sequences of all known CVEV isolates revealed that CVEV-DT1 was in an evolutionary branch with other isolates from China. Molecular variation analysis showed that the single nucleotide variability along CVEV full-length sequences was less than 8%, with more transitions (60.55%) than transversions (39.43%), indicating a genetically homogeneous CVEV population. In addition, non-synonymous nucleotide mutations mainly occurred in ORF1 and ORF2. Based on disorder analysis of all encoded ORF by CVEV-DT1, we identified that the CVEV-DT1 coat protein (CP) formed spherical granules, mainly in the cell nucleus and partly throughout the cytoplasm, with liquid properties through subcellular localization and photobleaching assay. Furthermore, we also confirmed that the CVEV P0 protein has weak post-transcriptional RNA-silencing suppressor activity and could elicit a strong hypersensitive response (HR) in tobacco plants. Collectively, to the best of our knowledge, our study was the first to profile the genomic variation in all the reported CVEV isolates and reveal the functions of CVEV-DT1-encoded proteins.
影响因子：
0.8
作者：
通讯作者：
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影响因子：
3.7
作者：
Dombrovsky A;Glanz E;Lachman O;Sela N;Doron-Faigenboim A;Antignus Y
通讯作者：
Antignus Y