The complete genomic sequence of pepper yellow leaf curl virus (PYLCV) and its implications for our understanding of evolution dynamics in the genus polerovirus.
The complete genomic sequence of pepper yellow leaf curl virus (PYLCV) and its implications for our understanding of evolution dynamics in the genus polerovirus.
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DOI：
10.1371/journal.pone.0070722
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Antignus Y
中科院分区：
文献类型：
--
作者：
Dombrovsky A;Glanz E;Lachman O;Sela N;Doron-Faigenboim A;Antignus Y
我们确定了一个假定的成员Polerovirus暂时命名为辣椒黄化曲叶病毒（PYLCV）的基因组的完整序列和组织。PYLCV的寄主范围比烟草脉曲病毒（TVDV）更广，并且与瓜蚜传黄化病毒（CABYV）（均为polerovirus）具有密切的血清学关系。对提取的病毒RNA进行SOLiD下一代序列分析，并用作逆转录合成的模板，随后进行PCR扩增。PYLCV的ssRNA基因组包括编码六个开放阅读框（ORF）的6，028个核苷酸，这是Polerovirus属的典型特征。将PYLCV ORF 2-4和ORF 5推导的氨基酸序列进行比较，发现它们与TVDV ORF 2-4和CABYV ORF 5的同源性分别为84-93%和87%。PYLCV和胡椒脉黄化病毒（PeVYV）都含有指向共同祖先polerovirus的序列。位于CABYV ORF 3编码病毒外壳蛋白（CP）的重组断裂点可能解释了在辣椒感染病毒PYLCV和PeVYV基因组中发现的CABYV样序列。在3' NCR中的核苷酸4，962和5，061（ORF 5）之间以及位置5，866和6，028之间鉴定了PYLCV特有的两个额外区域（PY 1和PY 2）。对感染胡椒的PeVYV的序列分析显示了三个独特的区域（Pe 1-Pe 3），与Polerovirus属的其他成员没有相似性。PYLCV和PeVYV的基因组分析表明，这些病毒的物种形成是通过共同感染共同宿主的polerovirus之间的推定重组事件发生的，导致PYLCV的出现，PYLCV是一种具有更广泛宿主范围的新型病原体。
We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV). PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV) and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV) (both poleroviruses). The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs), which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93%) and to ORF5 of CABYV (87%). Both PYLCV and Pepper vein yellowing virus (PeVYV) contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP), may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2) were identified between nucleotides 4,962 and 5,061 (ORF 5) and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3) with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s) between poleroviruses co-infecting a common host(s), resulting in the emergence of PYLCV, a novel pathogen with a wider host range.
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