Mutants in Drosophila TRPC channels reduce olfactory sensitivity to carbon dioxide.
Mutants in Drosophila TRPC channels reduce olfactory sensitivity to carbon dioxide.
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DOI：
10.1371/journal.pone.0049848
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hasan G
中科院分区：
文献类型：
--
作者：
Badsha F;Kain P;Prabhakar S;Sundaram S;Padinjat R;Rodrigues V;Hasan G
经典瞬时受体电位（TRPC）阳离子通道的成员在果蝇光感受器中的Gαq和PLCβ下游起作用，用于转导视觉刺激。Gαq最近被认为与二氧化碳（CO2）和其他气味的嗅觉传感有关。本文研究了果蝇PLCβ和TRPC通道在感受CO2中的作用。通过行为分析，它表明，果蝇突变体的plc 21 c，trp和trpl具有降低的敏感性CO2。TRP、TRPL和TRPγ的免疫组织化学染色表明，所有三个通道都在果蝇触角中表达，包括表达CO2受体的感觉神经元。从TRP（trp 343）和TRPL（trp 1302）的蛋白质无效等位基因的触角获得的电生理记录表明，对多个浓度的CO2的感觉反应降低。然而，trp 1302; trp 343双突变体仍然具有对CO2的残余响应。TRPC通道的下调，特别是在CO2敏感嗅觉神经元减少对CO2的反应，这种减少，即使在成年嗅觉感觉神经元的TRPC下调。因此，从TRPC RNAi菌株的触角获得的对CO2的响应降低不是由于发育缺陷。这些观察结果表明，TRPC通道功能的减少显着降低了嗅觉反应的敏感性，以5%或更低的CO2浓度在成年果蝇。推测Gr 63 a和Gr 21 a可能通过Gαq和PLC 21 C激活TRPC通道。
Members of the canonical Transient Receptor Potential (TRPC) class of cationic channels function downstream of Gαq and PLCβ in Drosophila photoreceptors for transducing visual stimuli. Gαq has recently been implicated in olfactory sensing of carbon dioxide (CO2) and other odorants. Here we investigated the role of PLCβ and TRPC channels for sensing CO2 in Drosophila. Through behavioral assays it was demonstrated that Drosophila mutants for plc21c, trp and trpl have a reduced sensitivity for CO2. Immuno-histochemical staining for TRP, TRPL and TRPγ indicates that all three channels are expressed in Drosophila antennae including the sensory neurons that express CO2 receptors. Electrophysiological recordings obtained from the antennae of protein null alleles of TRP (trp343) and TRPL (trpl302), showed that the sensory response to multiple concentrations of CO2 was reduced. However, trpl302; trp343 double mutants still have a residual response to CO2. Down-regulation of TRPC channels specifically in CO2 sensing olfactory neurons reduced the response to CO2 and this reduction was obtained even upon down-regulation of the TRPCs in adult olfactory sensory neurons. Thus the reduced response to CO2 obtained from the antennae of TRPC RNAi strains is not due to a developmental defect. These observations show that reduction in TRPC channel function significantly reduces the sensitivity of the olfactory response to CO2 concentrations of 5% or less in adult Drosophila. It is possible that the CO2 receptors Gr63a and Gr21a activate the TRPC channels through Gαq and PLC21C.
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