Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii.
Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii.
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DOI：
10.3389/fpls.2017.02154
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发表时间：
2017
影响因子：
5.6
通讯作者：
Huang F
中科院分区：
文献类型：
--
作者：
Xing J;Liu P;Zhao L;Huang F
绿藻莱茵衣藻是研究单细胞真核生物光合作用和氧化应激的关键模型生物。使用正向遗传学方法，我们鉴定并表征了突变体 x32，该突变体在正常和应激条件下缺乏 GreenCut2 中名为 CGLD1（绿色谱系和硅藻 1 中保守）的预测蛋白。我们发现，CGLD1 的缺失会导致生物体的光合自养生长和 PSII 活性降至最低。基于 blue-native (BN)/PAGE 和免疫印迹分析，我们观察到 x32 突变体中 PSII 复合物和核心亚基的数量减少。此外，x32表现出对高光胁迫的敏感性增加，以及对不同活性氧（ROS）胁迫处理的耐受性改变，即对H2O2/或叔丁基过氧化氢（t-BOOH）的耐受性降低，对中性红（NR）和玫瑰红（RB）的耐受性增加，分别诱导单线态氧的形成。通过定量实时 PCR (qRT-PCR) 的进一步分析表明，x32 增加的单线态氧耐受性在很大程度上与谷胱甘肽-S-转移酶 (GST) 基因表达的上调相关。我们的实验揭示的表型和生理学意义强调了 CGLD1 在维持 PSII 的结构和功能以及在光氧化应激条件下保护衣藻方面的重要作用。
The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. Using a forward genetics approach, we have identified and characterized a mutant x32, which lacks a predicted protein named CGLD1 (Conserved in Green Lineage and Diatom 1) in GreenCut2, under normal and stress conditions. We show that loss of CGLD1 resulted in minimal photoautotrophic growth and PSII activity in the organism. We observed reduced amount of PSII complex and core subunits in the x32 mutant based on blue-native (BN)/PAGE and immunoblot analysis. Moreover, x32 exhibited increased sensitivity to high-light stress and altered tolerance to different reactive oxygenic species (ROS) stress treatments, i.e., decreased resistance to H2O2/or tert-Butyl hydroperoxide (t-BOOH) and increased tolerance to neutral red (NR) and rose bengal (RB) that induce the formation of singlet oxygen, respectively. Further analysis via quantitative real-time PCR (qRT-PCR) indicated that the increased singlet-oxygen tolerance of x32 was largely correlated with up-regulated gene expression of glutathione-S-transferases (GST). The phenotypical and physiological implications revealed from our experiments highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas under photo-oxidative stress conditions.
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影响因子：
6.4
作者：
Berry, Lindsay L.;Brzezowski, Pawel;Wilson, Kenneth E.
通讯作者：
Wilson, Kenneth E.
DOI：
10.1073/pnas.1116843109
发表时间：
2012-05-15
影响因子：
11.1
作者：
Fischer, Beat B.;Ledford, Heidi K.;Niyogi, Krishna K.
通讯作者：
Niyogi, Krishna K.
DOI：
10.1111/tpj.13053
发表时间：
2015-12
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Blaby IK;Blaby-Haas CE;Pérez-Pérez ME;Schmollinger S;Fitz-Gibbon S;Lemaire SD;Merchant SS
通讯作者：
Merchant SS
影响因子：
--
作者：
Ledford, Heidi K.;Chin, Brian L.;Niyogi, Krishna K.
通讯作者：
Niyogi, Krishna K.
影响因子：
5.1
作者：
Leisinger, U;Rüfenacht, K;Eggen, RIL
通讯作者：
Eggen, RIL