Disparate effects of monensin and colchicine on intracellular processing of secretory proteins in cultured rat hepatocytes.
Disparate effects of monensin and colchicine on intracellular processing of secretory proteins in cultured rat hepatocytes.
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莫能菌素和秋水仙碱对培养的大鼠肝细胞中分泌蛋白的细胞内加工的不同影响。
DOI：
10.1111/j.1432-1033.1983.tb07639.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Y. Ikehara
中科院分区：
文献类型：
--
作者：
K. Oda;Y. Misumi;Y. Ikehara
我们研究了培养的大鼠肝细胞中三种主要分泌蛋白--白蛋白、α1-蛋白酶抑制物和α2U-球蛋白的生物合成和胞内加工。测定了分泌阻滞剂、单价离子载体莫能菌素以及微管影响剂秋水仙碱和紫杉醇的作用。在对照细胞中，α1-蛋白酶抑制剂是一种糖蛋白，首先合成对内糖苷酶敏感的形式，其相对分子质量为51000，然后加工成两种对内糖苷酶敏感的形式，其相对分子质量分别为51000和56000，后者被分泌到培养液中。最初合成的前白蛋白被Chase转化为血清型白蛋白，而没有鉴定出α2U球蛋白的前型前体。在秋水仙碱或紫杉醇处理的细胞中，分泌受到极大抑制的细胞中，完全加工的α1-蛋白酶抑制剂和白蛋白积累并最终分泌到培养液中。然而，在莫能菌素处理的细胞中，大多数新合成的α1-蛋白酶抑制剂和白蛋白没有加工成最终的成熟形式，导致两种51000-MR形式和前白蛋白分别积累。此外，在处理过的细胞中，前白蛋白和内切糖苷酶-H抗性的α1-蛋白酶抑制剂最终被分泌到培养液中。这种作用不是由NH4Cl引起的，NH4Cl也抑制分泌，并已知在受体介导的内吞作用途径上发挥与莫能菌素类似的作用。基于这些结果，莫能菌素的使用可能被证明对于更详细地分析各种蛋白质的细胞内过程是有价值的。
We have studied the biosynthesis and intracellular processing of three major secretory proteins, albumin, alpha 1-protease inhibitor and alpha 2u-globulin, in cultured rat hepatocytes. The effect of secretion-blocking agents, monensin, a monovalent ionophore, and the microtubule-affecting agents colchicine and taxol was determined. In the control cells, alpha 1-protease inhibitor, a glycoprotein, was first synthesized as an endoglycosidase-H-sensitive form with Mr 51 000, and then processed to two endoglycosidase-H-resistant forms having Mr 51 000 and 56 000, the latter of which was secreted into the medium. Initially synthesized proalbumin was converted with chase to serum-type albumin, while no pro-type precursor was identified for alpha 2u-globulin. In the cells treated with colchicine or taxol, in which secretion was greatly inhibited, the fully processed alpha 1-protease inhibitor and albumin accumulated and were finally secreted into the medium. In the monensin-treated cells, however, most of the newly synthesized alpha 1-protease inhibitor and albumin were not processed to the final mature forms, resulting in accumulation of two 51 000-Mr forms and proalbumin, respectively. Moreover in treated cells, proalbumin and the endoglycosidase-H-resistant alpha 1-protease inhibitor were finally secreted into the medium. Such an effect was not caused by NH4Cl which also inhibited the secretion and is known to exert the similar effect as monensin on the receptor-mediated endocytosis pathway. Based on these results, the use of monensin may prove valuable for more detailed analysis of intracellular processing of various proteins.
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影响因子：
56.9
作者：
ROTHMAN, JE
通讯作者：
ROTHMAN, JE
莫能菌素存在时分泌的人纤连蛋白糖基化异常。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ledger,PW;Nishimoto,SK;Hayashi,S;Tanzer,ML
通讯作者：
Tanzer,ML
DOI：
10.1016/s0021-9258(18)34831-2
发表时间：
1982-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Tulsiani;S C Hubbard;P. W. Robbins;O. Touster
通讯作者：
D. Tulsiani;S C Hubbard;P. W. Robbins;O. Touster
DOI：
10.1073/pnas.79.17.5297
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARSH, M;WELLSTEED, J;HELENIUS, A
通讯作者：
HELENIUS, A
大鼠肝α2u-球蛋白的前体：其信号肽的部分氨基酸序列测定。
影响因子：
2.7
作者：
Ikehara,Y;Oda,K;Rosenfeld,MG;Bar-Nun,S;Kreibich,G
通讯作者：
Kreibich,G