LncRNA Airsci increases the inflammatory response after spinal cord injury in rats through the nuclear factor kappa B signaling pathway.
LncRNA Airsci increases the inflammatory response after spinal cord injury in rats through the nuclear factor kappa B signaling pathway.
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LncRNA Airsci通过核因子κB信号通路增加大鼠脊髓损伤后的炎症反应
DOI：
10.4103/1673-5374.295335
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发表时间：
2021-04
影响因子：
6.1
通讯作者：
Lv CL
中科院分区：
文献类型：
--
作者：
Zhang T;Li K;Zhang ZL;Gao K;Lv CL
脊髓损伤是一种严重的中枢神经系统创伤性事件。研究表明，长链非编码rna （long non-coding RNAs, lncRNAs）在脊髓损伤急性期的炎症反应调控中发挥重要作用。在这里，我们研究了一个新的与脊髓损伤和急性炎症相关的lncRNA。我们分析了脊髓损伤后lncRNA的表达谱，并探讨了lncRNA Airsci（脊髓急性炎症反应）在急性脊髓损伤后恢复中的作用。将大鼠分为对照组、SCI组和SCI + lncRNA Airsci-siRNA组。western blot法检测脊髓损伤后1 ~ 28 d大鼠核因子κB [NF-κB (p65)]、NF-κB抑制剂i -κB α、磷酸化i -κB α (p -κB α)表达及p- i -κB α/ i -κB α比值。通过RNA测序评估脊髓损伤后lncRNA的差异表达谱。利用生物信息学技术对差异表达的lncrna进行分析。通过实时荧光定量PCR验证了参与NF-κB信号通路并与急性炎症反应相关的lncRNA Airsci的差异表达。采用western blot法和实时荧光定量PCR法检测脊髓损伤后3 d的白细胞介素（IL-1β）、IL-6和肿瘤坏死因子（TNF-α）水平。采用苏木精-伊红染色和尼氏染色评价脊髓组织病理学。采用Basso， Beattie和Bresnahan运动评定量表评估运动功能。在脊髓损伤后检测到大量差异表达的lncrna，与对照组相比，脊髓损伤3 d组有151个lncrna表达上调，186个lncrna表达下调。在参与NF-κB信号通路的5个LncRNA中，LncRNA Airsci的表达最为显著。LncRNA Airsci-siRNA通过抑制NF-κB信号通路降低炎症反应，减轻脊髓组织损伤，促进脊髓损伤大鼠运动功能恢复。这些发现表明脊髓损伤后大量lncRNA存在差异表达，抑制lncRNA Airsci可通过NF-κB信号通路减少炎症反应，从而促进功能恢复。所有实验程序和方案经济宁医学院动物伦理委员会批准(批准号：JNMC-2020-DW-RM-003)于2020年1月18日发布。
Spinal cord injury (SCI) is a serious traumatic event to the central nervous system. Studies show that long non-coding RNAs (lncRNAs) play an important role in regulating the inflammatory response in the acute stage of SCI. Here, we investigated a new lncRNA related to spinal cord injury and acute inflammation. We analyzed the expression profile of lncRNAs after SCI, and explored the role of lncRNA Airsci (acute inflammatory response in SCI) on recovery following acute SCI. The rats were divided into the control group, SCI group, and SCI + lncRNA Airsci-siRNA group. The expression of inflammatory factors, including nuclear factor kappa B [NF-κB (p65)], NF-κB inhibitor IκBα and phosphorylated IκBα (p-IκBα), and the p-IκBα/IκBα ratio were examined 1–28 days after SCI in rats by western blot assay. The differential lncRNA expression profile after SCI was assessed by RNA sequencing. The differentially expressed lncRNAs were analyzed by bioinformatics technology. The differentially expressed lncRNA Airsci, which is involved in NF-κB signaling and associated with the acute inflammatory response, was verified by quantitative real-time PCR. Interleukin (IL-1β), IL-6 and tumor necrosis factor (TNF-α) at 3 days after SCI were measured by western blot assay and quantitative real-time PCR. The histopathology of the spinal cord was evaluated by hematoxylin-eosin and Nissl staining. Motor function was assessed with the Basso, Beattie and Bresnahan Locomotor Rating Scale. Numerous differentially expressed lncRNAs were detected after SCI, including 151 that were upregulated and 186 that were downregulated in the SCI 3 d group compared with the control group. LncRNA Airsci was the most significantly expressed among the five lncRNAs involved in the NF-κB signaling pathway. LncRNA Airsci-siRNA reduced the inflammatory response by inhibiting the NF-κB signaling pathway, alleviated spinal cord tissue injury, and promoted the recovery of motor function in SCI rats. These findings show that numerous lncRNAs are differentially expressed following SCI, and that inhibiting lncRNA Airsci reduces the inflammatory response through the NF-κB signaling pathway, thereby promoting functional recovery. All experimental procedures and protocols were approved by the approved by the Animal Ethics Committee of Jining Medical University (approval No. JNMC-2020-DW-RM-003) on January 18, 2020.
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DOI：
10.21037/jss.2017.10.06
发表时间：
2017-12-01
期刊：
Journal of spine surgery (Hong Kong)
影响因子：
--
作者：
Cheng, Ivan;Park, Don Y;Kharazi, Alexander I
通讯作者：
Kharazi, Alexander I
影响因子：
5.3
作者：
Huang, Li-Jun;Li, Ge;Zeng, Yuan-Shan
通讯作者：
Zeng, Yuan-Shan
影响因子：
9.3
作者：
Wang, Chao;Zhang, Lu;Liu, Chuan-ju
通讯作者：
Liu, Chuan-ju
影响因子：
2.8
作者：
Kleene, Ralf;Loers, Gabriele;Schachner, Melitta
通讯作者：
Schachner, Melitta
影响因子：
6.1
作者：
Oh JY;Hwang TY;Jang JH;Park JY;Ryu Y;Lee H;Park HJ
通讯作者：
Park HJ