Interplay between oxygen and Fe-S cluster biogenesis: insights from the Suf pathway.
Interplay between oxygen and Fe-S cluster biogenesis: insights from the Suf pathway.
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DOI：
10.1021/bi500488r
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发表时间：
2014-09-23
期刊：
影响因子：
2.9
通讯作者：
Outten FW
中科院分区：
文献类型：
--
作者：
Boyd ES;Thomas KM;Dai Y;Boyd JM;Outten FW
铁硫（Fe-S）簇金属蛋白在几乎所有当代生命形式中起着重要作用。铁- s团簇几乎无处不在的存在以及对铁- s团簇的基本需求在好氧和厌氧古菌、细菌和真核生物中都存在，这表明这些团簇可能在地球大气广泛氧化之前的生命进化早期就被整合到中心代谢途径中。有趣的是，由于铁的生物利用度降低以及硫转运中间体和铁- s团簇被活性氧直接氧化，铁- s团簇依赖的代谢对氧的破坏很敏感。这一事实，再加上好氧生物中无处不在的Fe-S簇，表明生物进化具有促进生物发生和在氧气存在下使用这些必需辅因子的机制，这些辅因子在大约25亿年前随着含氧光合作用的增殖和抗氧化的减少矿物质的耗尽而逐渐开始积累。这篇综述强调了Fe-S簇生物发生途径中最古老的Suf系统，它可能存在于早期厌氧生命形式中。在此，我们利用Suf通路的进化来评估Suf蛋白的生化功能和生理作用之间的关系，重点是氧毒性的选择压力。我们的分析表明，进入含氧环境的多样化破坏了铁和硫的代谢，并且是核心SufB-SufC支架复合物获得辅助Suf蛋白（如SufD， SufE和SufS）的主要驱动力。该分析为研究含铁硫团簇生物发生途径和含铁硫团簇金属酶及其对氧响应的复杂发散模式提供了新的框架。
Iron–sulfur (Fe–S) cluster metalloproteins conduct essential functions in nearly all contemporary forms of life. The nearly ubiquitous presence of Fe–S clusters and the fundamental requirement for Fe–S clusters in both aerobic and anaerobic Archaea, Bacteria, and Eukarya suggest that these clusters were likely integrated into central metabolic pathways early in the evolution of life prior to the widespread oxidation of Earth’s atmosphere. Intriguingly, Fe–S cluster-dependent metabolism is sensitive to disruption by oxygen because of the decreased bioavailability of ferric iron as well as direct oxidation of sulfur trafficking intermediates and Fe–S clusters by reactive oxygen species. This fact, coupled with the ubiquity of Fe–S clusters in aerobic organisms, suggests that organisms evolved with mechanisms that facilitate the biogenesis and use of these essential cofactors in the presence of oxygen, which gradually began to accumulate around 2.5 billion years ago as oxygenic photosynthesis proliferated and reduced minerals that buffered against oxidation were depleted. This review highlights the most ancient of the Fe–S cluster biogenesis pathways, the Suf system, which likely was present in early anaerobic forms of life. Herein, we use the evolution of the Suf pathway to assess the relationships between the biochemical functions and physiological roles of Suf proteins, with an emphasis on the selective pressure of oxygen toxicity. Our analysis suggests that diversification into oxygen-containing environments disrupted iron and sulfur metabolism and was a main driving force in the acquisition of accessory Suf proteins (such as SufD, SufE, and SufS) by the core SufB–SufC scaffold complex. This analysis provides a new framework for the study of Fe–S cluster biogenesis pathways and Fe–S cluster-containing metalloenzymes and their complicated patterns of divergence in response to oxygen.
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影响因子：
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