Blood Group A Glycosyltransferase Occurring as Alleles with High Sequence Difference Is Transiently Induced during aNippostrongylus brasiliensis Parasite Infection*
Blood Group A Glycosyltransferase Occurring as Alleles with High Sequence Difference Is Transiently Induced during aNippostrongylus brasiliensis Parasite Infection*
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在巴西圆线虫寄生虫感染过程中，短暂诱导存在高序列差异等位基因的 A 血型糖基转移酶*
DOI：
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发表时间：
2002
影响因子：
4.8
通讯作者：
N. Karlsson
中科院分区：
文献类型：
--
作者：
F. Olson;M. Johansson;K. Klinga;D. Bouhours;L. Enerbäck;G. Hansson;N. Karlsson
释放来自对照大鼠和感染巴西圆线虫寄生虫的大鼠小肠的中性粘蛋白寡糖，并通过气相色谱-质谱法进行分析。受感染的动物表达了七种类似A型血的结构，而对照组动物则没有这些结构。这些表位的血型 A 性质通过制备的粘蛋白的 A 型血反应性得到证实，其中 Muc2 是其中之一。对受感染动物的小肠进行的转移酶测定和 Northern 印迹表明，已诱导出与人 A 血型糖基转移酶相似的 α-N-乙酰半乳糖胺基转移酶。该表达是一个短暂事件，在 13 天感染的第 6 天达到最大值。克隆了大鼠血型 A 糖基转移酶，显示出两种形式，氨基酸相似性达 95%。两种类型都具有 A 型血转移酶活性，并且可能是等位基因，因为分析的 12 个近交系中没有一个携带这两种类型。第二种类型是在远交大鼠和一种近交品系中发现的。每种类型的近交大鼠的第一代后代都是杂合的，进一步支持了等位基因假说。瞬时诱导和大的等位基因变异可能表明糖基转移酶是改变粘蛋白和其他糖缀合物的动态系统的一部分，作为对抗微生物挑战的保护机制。
Neutral mucin oligosaccharides from the small intestine of control rats and rats infected with the parasiteNippostrongylus brasiliensis were released and analyzed by gas chromatography-mass spectrometry. Infected animals expressed seven blood group A-like structures that were all absent in the control animals. The blood group A nature of these epitopes was confirmed by blood group A reactivity of the prepared mucins, of which Muc2 was one. Transferase assays and Northern blotting on small intestines from infected animals showed that an α-N-acetylgalactosaminyltransferase similar to the human blood group A glycosyltransferase had been induced. The expression was a transient event, with a maximum at day 6 of the 13-day-long infection. The rat blood group A glycosyltransferase was cloned, revealing two forms with an amino acid similarity of 95%. Both types had blood group A transferase activity and were probably allelic because none of 12 analyzed inbred strains carried both types. The second type was found in outbred rats and in one inbred strain. First generation offspring of inbred rats of each type were heterozygous, further supporting the allelic hypothesis. The transient induction and the large allelic variation could suggest that glycosyltransferases are part of a dynamic system altering mucins and other glycoconjugates as a protecting mechanism against microbial challenges.
DOI：
10.1016/s0021-9258(19)40170-1
发表时间：
1990-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Yamamoto;J. Marken;T. Tsuji;T. White;H. Clausen;S. Hakomori
通讯作者：
F. Yamamoto;J. Marken;T. Tsuji;T. White;H. Clausen;S. Hakomori
影响因子：
4.3
作者：
YAMAMOTO, F;MCNEILL, PD;HAKOMORI, S
通讯作者：
HAKOMORI, S
DOI：
--
发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
F. Yamamoto;S. Hakomori
通讯作者：
F. Yamamoto;S. Hakomori