MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function.
MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function.
复制标题
DOI：
10.1016/j.isci.2023.106339
复制
发表时间：
2023-04-21
期刊：
影响因子：
5.8
通讯作者：
Forde, Niamh
中科院分区：
文献类型：
--
作者：
Hume, Laura;Edge, Jessica C.;Tinning, Haidee;Wang, Dapeng;Taylor, Alysha S.;Ovchinnikov, Vladimir;Geijer-Simpson, Annika, V;Vrljicak, Pavle;Brosens, Jan J.;Lucas, Emma S.;Simpson, Nigel A. B.;Shillito, Jayne;Forbes, Karen;O'Connell, Mary J.;Forde, Niamh
我们测试了以下假设：保守的胎盘哺乳动物特异性 microRNA 及其靶标促进子宫内膜对植入的容受性。 miR-340-5p、-542-3p 和 -671-5p 的表达通过将子宫内膜上皮细胞暴露于黄体酮 (10 μg/ml) 24 小时与 1,713 个预测靶点协调来调节。对转染 miRNA 模拟物/抑制剂 (48 h: n = 3) 的细胞进行蛋白质组学分析，结果显示 1,745 个蛋白质被 miR-340-5p 改变（模拟物；1,369，抑制剂；376），其中 171 个是预测靶标并受 P4 调节。 MiR-542-3p 改变了 2,353 个（模拟；1,378，抑制剂；975）100 个，这是 mirDB 预测的，包括 46 个 P4 调节的。 MiR-671-5p 改变了 1,744 个蛋白质（模拟蛋白；1,252 个，抑制剂蛋白；492 个），其中 95 个是预测靶标，46 个是 P4 调节的。无论妊娠结局如何，所有 miRNA 均在黄体期子宫内膜活检中检测到。与随后活产的个体相比，先前和随后流产的个体的活检中 miR-340-5p 表达增加。这些 miRNA 及其靶标的功能障碍会导致子宫内膜源性复发性流产。黄体酮上调子宫内膜上皮中的 miR-340-5p、-542-3p 和 -671-5p 黄体酮改变参与增殖和上皮发育的转录物 过度表达或表达不足这些 microRNA 会导致蛋白质丰度改变 改变的蛋白质的功能涉及着床和子宫容受性分子生物学；发育生物学；胚胎学
We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 μg/ml) for 24 h coordinate with 1,713 of their predicted targets. Proteomic analysis of cells transfected with miRNA mimic/inhibitor (48 h: n = 3) revealed 1,745 proteins altered by miR-340-5p (mimic; 1,369, inhibitor; 376) of which 171 were predicted targets and P4-regulated. MiR-542-3p altered 2,353 (mimic; 1,378, inhibitor; 975) 100 which were mirDB predicted, including 46 P4-regulated. MiR-671-5p altered 1,744 proteins (mimic; 1,252, inhibitor; 492) 95 of which were predicted targets and 46 P4-regulated. All miRNAs were detected in luteal phase endometrial biopsies, irrespective of pregnancy outcomes. miR-340-5p expression increased in biopsies from individuals suffering previous and subsequent miscarriage compared to those with subsequent live birth. Dysfunction of these miRNAs and their targets contribute to endometrial-derived recurrent pregnancy loss. Progesterone upregulates miR-340-5p, -542-3p and -671-5p in endometrial epithelium Progesterone alters transcripts involved in proliferation and epithelial development Over- or under-expressing these microRNAs results in altered abundance of proteins Functions of altered proteins are involved in implantation and uterine receptivity Biological sciences; Molecular biology; Developmental biology; Embryology
登录
查看更多内容
影响因子：
14.9
作者：
Frankish A;Diekhans M;Ferreira AM;Johnson R;Jungreis I;Loveland J;Mudge JM;Sisu C;Wright J;Armstrong J;Barnes I;Berry A;Bignell A;Carbonell Sala S;Chrast J;Cunningham F;Di Domenico T;Donaldson S;Fiddes IT;García Girón C;Gonzalez JM;Grego T;Hardy M;Hourlier T;Hunt T;Izuogu OG;Lagarde J;Martin FJ;Martínez L;Mohanan S;Muir P;Navarro FCP;Parker A;Pei B;Pozo F;Ruffier M;Schmitt BM;Stapleton E;Suner MM;Sycheva I;Uszczynska-Ratajczak B;Xu J;Yates A;Zerbino D;Zhang Y;Aken B;Choudhary JS;Gerstein M;Guigó R;Hubbard TJP;Kellis M;Paten B;Reymond A;Tress ML;Flicek P
通讯作者：
Flicek P
影响因子：
14.9
作者：
Fromm, Bastian;Domanska, Diana;Peterson, Kevin J.
通讯作者：
Peterson, Kevin J.
影响因子：
3.6
作者：
Bounds KR;Chiasson VL;Pan LJ;Gupta S;Chatterjee P
通讯作者：
Chatterjee P
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
3.4
作者：
Hosseini MK;Gunel T;Gumusoglu E;Benian A;Aydinli K
通讯作者：
Aydinli K