Construction of a panel of glucose indicator proteins for continuous glucose monitoring.
Construction of a panel of glucose indicator proteins for continuous glucose monitoring.
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DOI：
10.1016/j.bios.2011.01.017
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发表时间：
2011-04-15
影响因子：
12.6
通讯作者：
Ye K
中科院分区：
文献类型：
--
作者：
Jin S;Veetil JV;Garrett JR;Ye K
The development of implantable glucose sensors for use in diabetes treatment has been pursued for decades. However, enzyme-based glucose sensors often fail in vivo. In our previous work, we engineered a novel glucose indicator protein (GIP) that can sense glucose without relying on any enzymes and cofactors. Nevertheless, this GIP is unsuitable for blood glucose monitoring due to its low dissociation constant. Here, we report a novel approach to creating a new GIP that can be used to monitor blood glucose level. By disrupting pi-pi stacking around GIP’s glucose binding site through site-directed mutagenesis, we showed that GIP’s dissociation constant can be manipulated from 0.026 mM to 7.86 mM. This approach yielded four GIP mutants. We showed that one of the mutants can be used to detect glucose from 0 to 32 mM, while another mutant can be employed to visualize intracellular glucose (0–200 μM) within living cells through FRET imaging microscopy measurement.
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影响因子：
12.6
作者：
Veetil JV;Jin S;Ye K
通讯作者：
Ye K
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通讯作者：
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Makhatadze, GI
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