A glucose sensor protein for continuous glucose monitoring.
A glucose sensor protein for continuous glucose monitoring.
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DOI：
10.1016/j.bios.2010.08.052
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发表时间：
2010-12-15
影响因子：
12.6
通讯作者：
Ye K
中科院分区：
文献类型：
--
作者：
Veetil JV;Jin S;Ye K
由于缺乏可靠的无创或至少是微创技术，体内连续血糖监测对葡萄糖传感器的发展提出了重大挑战。在这项概念验证研究中，我们展示了一种新的葡萄糖传感器蛋白AcGFP1-GBPcys-mCherry的开发，以及一种光学传感器组件，能够产生可量化的FRET（荧光共振能量转移）信号，用于葡萄糖监测。我们的实验数据表明，工程葡萄糖传感器蛋白可以产生可测量的FRET信号，以响应葡萄糖浓度从25到800 μM。基于这种蛋白质开发的传感器具有长达3周的保质期。当在这些化合物的生理显著浓度下进行测试时，传感器的反应没有受到半乳糖、果糖、乳糖、甘露糖和甘露醇等化合物的干扰。这种新的葡萄糖传感器蛋白有可能用于开发可植入的葡萄糖传感器，用于连续血糖监测。
In vivo continuous glucose monitoring has posed a significant challenge to glucose sensor development due to the lack of reliable techniques that are non-or at least minimally-invasive. In this proof-of-concept study, we demonstrated the development of a new glucose sensor protein, AcGFP1-GBPcys-mCherry, and an optical sensor assembly, capable of generating quantifiable FRET (fluorescence resonance energy transfer) signals for glucose monitoring. Our experimental data showed that the engineered glucose sensor protein can generate measureable FRET signals in response to glucose concentrations varying from 25 to 800 μM. The sensor developed based on this protein had a shelf life of up to 3 weeks. The sensor response was devoid of interference from compounds like galactose, fructose, lactose, mannose, and mannitol when tested at physiologically significant concentrations of these compounds. This new glucose sensor protein can potentially be used to develop implantable glucose sensors for continuous glucose monitoring.
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