Cross-talk between light and glucose regulation controls toxin production and morphogenesis in Aspergillus nidulans.
Cross-talk between light and glucose regulation controls toxin production and morphogenesis in Aspergillus nidulans.
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DOI：
10.1016/j.fgb.2010.08.007
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发表时间：
2010-12
影响因子：
3
通讯作者：
Calvo, A. M.
中科院分区：
文献类型：
--
作者：
Atoui, A.;Kastner, C.;Larey, C. M.;Thokala, R.;Etxebeste, O.;Espeso, E. A.;Fischer, R.;Calvo, A. M.
关键词：
光是一种主要的环境刺激，对生物体有广泛的影响，触发细胞反应，导致最佳适应，提高适应性和生存。在真菌中，光影响生长，并引起各种形态变化，如导致繁殖的变化。光也可以影响真菌的新陈代谢，包括天然产物的生物合成。在这项研究中，我们表明，在构巢曲霉的杂色曲霉素（ST）毒素的生产光的效果取决于葡萄糖浓度。在1%的葡萄糖和光照下生长的培养物中，ST产量低于在黑暗中生长时。这种较低的ST生产与细胞损伤率升高相一致，细胞核完整性部分丧失，细胞质空泡化。然而，在2%的葡萄糖培养这些影响被逆转，光增强ST生产。葡萄糖的丰度也影响了光依赖的亚细胞定位的VeA（天鹅绒）蛋白，一个关键的调节器所需的正常光依赖的形态发生和次生代谢的真菌和其他真菌属。其他VeA相关蛋白，特别是蓝光敏感蛋白LreA和LreB（WC-1和WC-2直系同源物），对分生孢子的作用也可以通过丰富的葡萄糖来修改。我们还表明，LreA和LreB，以及光敏色素FphA，调制不仅杂色曲霉素的合成，但也生产的抗生素青霉素。
Light is a major environmental stimulus that has a broad effect on organisms, triggering a cellular response that results in an optimal adaptation enhancing fitness and survival. In fungi, light affects growth, and causes diverse morphological changes such as those leading to reproduction. Light can also affect fungal metabolism, including the biosynthesis of natural products. In this study we show that in Aspergillus nidulans the effect of light on the production of the sterigmatocystin (ST) toxin depends on the glucose concentration. In cultures grown with 1% glucose and exposed to light, ST production was lower than when grown in the dark. This lower ST production coincided with an elevated rate of cellular damage with partial loss of nuclear integrity and vacuolated cytoplasm. However, in cultures grown with 2% glucose these effects were reversed and light enhanced ST production. Glucose abundance also affected the light-dependent subcellular localization of the VeA (velvet) protein, a key regulator necessary for normal light-dependent morphogenesis and secondary metabolism in Aspergilli and other fungal genera. The role of other VeA-associated proteins, particularly the blue light-sensing proteins LreA and LreB (WC-1 and WC-2 orthologs), on conidiation could also be modified by the abundance of glucose. We also show that LreA and LreB, as well as the phytochrome FphA, modulate not only the synthesis of sterigmatocystin, but also the production of the antibiotic penicillin.
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DOI：
10.1111/j.1432-1033.1975.tb03958.x
发表时间：
1975-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BAILEY, C;ARST, HN
通讯作者：
ARST, HN
影响因子：
5.3
作者：
DOWZER, CEA;KELLY, JM
通讯作者：
KELLY, JM
影响因子：
5.3
作者：
Belli, N.;Ramos, A. J.;Marin, S.
通讯作者：
Marin, S.
影响因子：
3.6
作者：
Fernandes, M;Keller, NP;Adams, TH
通讯作者：
Adams, TH
影响因子：
2.4
作者：
BUCHANAN, RL;STAHL, HG
通讯作者：
STAHL, HG