Physical mapping of the pink-eyed dilution complex in mouse chromosome 7 shows that Atp10c is the only transcript between Gabrb3 and Ube3a.

Physical mapping of the pink-eyed dilution complex in mouse chromosome 7 shows that Atp10c is the only transcript between Gabrb3 and Ube3a.
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小鼠 7 号染色体中红眼稀释复合物的物理图谱显示 Atp10c 是 Gabrb3 和 Ube3a 之间的唯一转录本。

DOI:
10.1080/10425170412331279855
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发表时间:
2004
期刊:
DNA sequence : the journal of DNA sequencing and mapping
影响因子:
--
通讯作者:
Johnson,DabneyK
Johnson,DabneyK
中科院分区:
--
文献类型:
--
作者:
Dhar,MadhuS;Hauser,LorenJ;Nicholls,RobertD;Johnson,DabneyK

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对一组在pink-eyed dilution(p)位点上的同源致死性缺失突变体的表型分析,鉴定出p-连锁肥胖位点1(plo 1),位于plocus的远端,是参与调节这些小鼠体内脂肪和/或影响脂质代谢的位点。plo 1区域映射到两个基因Gabrb 3和Ube 3a之间的小鼠7号染色体(MMU 7),这两个基因已被用作锚点来生成plo 1的整合缺失和物理图谱,该图谱包含约1.2-1.3 Mb。构建缺失/物理图谱,并对两个基因座之间的基因组DNA进行测序以鉴定定位到该区域的基因。数据表明,Atp 10 c,一种新的IV型ATP酶,一种假定的磷脂转运蛋白,是该染色体区域的唯一编码单位。
Phenotypic analyses of a set of homozygous-lethal deletion mutants at thepink-eyed dilution(p) locus has resulted in the identification ofp-linked obesity locus 1(plo 1), distal to theplocus, as a locus involved in the modulation of body fat and/or affecting lipid metabolism in these mice. Theplo 1region maps to mouse chromosome 7 (MMU 7) between two genes,Gabrb3andUbe3a, which have been used as anchor points to generate an integrated deletion and physical map ofplo 1that encompasses about 1.2-1.3 Mb. A deletion/physical map was constructed and the genomic DNA between the two loci was sequenced to identify genes mapping to this region. Data show thatAtp10c, a novel type IV ATPase a putative phospholipid transporter, is the only coding unit in this region of the chromosome.
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