Distant upstream regulatory sequences control the level of expression of the am (GDH) locus of Neurospora crassa.
Distant upstream regulatory sequences control the level of expression of the am (GDH) locus of Neurospora crassa.
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远端上游调控序列控制粗糙脉孢菌 am (GDH) 基因座的表达水平。
DOI：
10.1007/bf00321115
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发表时间：
1990
期刊：
影响因子：
2.5
通讯作者：
Kinsey,JA
中科院分区：
文献类型：
--
作者：
Frederick,GD;Kinsey,JA
We have constructed deletions in the 5′ non-coding sequences of the clonedNeurospora crassa amgene. Vectors with a truncated fragment of theamgene were used in transformation experiments to introduce the deletions into the chromosome by homologous recombination. Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression. The closer of these two elements (URSamα) is at approximately 1.4 kb upstream of the transcriptional start site. The second elements (URSamβ) is located between 2.1 and 3.2 kb upstream of the transcription start site. Deletion of either of these two elements reducesamexpression to about 50% of the wild-type level. Deletion of both elements reduceamexpression to from 5–16% of the wild-type level. Deletion of 1.1 kb of sequence just downstream of URSamα, which brings this element to within 300 bp of the transcription start site, had no effect onamexpression. Likewise, deletion of 3.5 kb of sequence upstream of URSamβ had no effect on expression. None of these deletions had any effect on the expression ofusg-1, a gene of unknown function that is transcribed in the same direction as theamgene, and which terminates about 3.5 kb upstream of the URSamβ element.
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