Identification of potential microRNAs and KEGG pathways in denervation muscle atrophy based on meta-analysis.
Identification of potential microRNAs and KEGG pathways in denervation muscle atrophy based on meta-analysis.
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DOI：
10.1038/s41598-021-92489-1
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发表时间：
2021-06-30
影响因子：
4.6
通讯作者：
Yin X
中科院分区：
文献类型：
--
作者：
Gu X;Jin B;Qi Z;Yin X
关于肌肉萎缩的分子机制已经有了很多研究，但对于失神经肌肉萎缩还没有全面的分析。控制失神经肌肉萎缩发展的基因网络需要进一步阐明。我们研究了来自五个失神经肌肉萎缩微阵列数据集的差异表达基因（DEG），并预测了靶向这些DEG的microRNA。我们还纳入了先前研究中失神经肌肉萎缩的差异表达microRNA数据集作为背景信息，以识别潜在的关键microRNA。最后，我们将失神经肌肉萎缩与其他原因引起的废用性肌肉萎缩进行比较，获得了仅在失神经肌肉萎缩中差异表达的Den-mRNA。在这项荟萃分析中，我们获得了失神经肌肉萎缩中429个上调基因，525个下调基因和一批关键microRNA。我们发现了8种重要的microRNA-mRNA相互作用（miR-1/Jun、miR-1/Vegfa、miR-497/Vegfa、miR-23 a/Vegfa、miR-206/Vegfa、miR-497/Suclg 1、miR-27 a/Suclg 1、miR-27 a/Mapk 14）。Den-gene富集的KEGG信号通路前5位分别是胰岛素信号通路、T细胞受体信号通路、MAPK信号通路、Toll样受体信号通路和B细胞受体信号通路。我们的研究揭示了失神经肌肉萎缩的RNA调控网络，揭示了失神经肌肉萎缩的特异性基因和术语。
The molecular mechanism of muscle atrophy has been studied a lot, but there is no comprehensive analysis focusing on the denervated muscle atrophy. The gene network that controls the development of denervated muscle atrophy needs further elucidation. We examined differentially expressed genes (DEGs) from five denervated muscle atrophy microarray datasets and predicted microRNAs that target these DEGs. We also included the differentially expressed microRNAs datasets of denervated muscle atrophy in previous studies as background information to identify potential key microRNAs. Finally, we compared denervated muscle atrophy with disuse muscle atrophy caused by other reasons, and obtained the Den-genes which only differentially expressed in denervated muscle atrophy. In this meta-analysis, we obtained 429 up-regulated genes, 525 down-regulated genes and a batch of key microRNAs in denervated muscle atrophy. We found eight important microRNA-mRNA interactions (miR-1/Jun, miR-1/Vegfa, miR-497/Vegfa, miR-23a/Vegfa, miR-206/Vegfa, miR-497/Suclg1, miR-27a/Suclg1, miR-27a/Mapk14). The top five KEGG pathways enriched by Den-genes are Insulin signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway and B cell receptor signaling pathway. Our research has delineated the RNA regulatory network of denervated muscle atrophy, and uncovered the specific genes and terms in denervated muscle atrophy.
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