Creation of a New Explosive Injury Equipment to Induce a Rabbit Animal Model of Closed Globe Blast Injury via Gas Shock.
Creation of a New Explosive Injury Equipment to Induce a Rabbit Animal Model of Closed Globe Blast Injury via Gas Shock.
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一种新型爆炸致伤装置的气激致兔封闭式爆炸伤动物模型的研制
DOI：
10.3389/fmed.2021.749351
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发表时间：
2021
影响因子：
3.9
通讯作者：
Yan H
中科院分区：
文献类型：
--
作者：
Liu Y;Yang T;Yu J;Li M;Li J;Yan H
应用自行研制的爆炸伤装置，建立兔闭合性地球仪爆炸伤动物模型，探讨不同气压下眼球的解剖及病理变化。该装置由高压气源压缩泵、气路、气震组成。有36只健康的蓝蓝色家兔暴露于5种爆炸压力（500、1，000、1，500、2，000和5，000 Kpa）中的一种。分别于染毒后0、1、3、7 d行裂隙灯显微镜、B超、眼底照相、光学相干断层扫描（OCT）、眼压（IOP）检查，7 d行大体组织病理学检查。对侧眼和非冲击波暴露兔用作对照。获得了闭合性地球仪爆炸伤的证据。在所有压力下的模型中观察到角膜水肿和前房出血，没有全层地球仪损伤或透镜破裂，因为严重程度与压力无关。B超和OCT检查均未见明显视网膜异常，但在5,000 Kpa暴露组中，1只眼分别出现轻度玻璃体积血、视网膜震荡和重度视网膜色素沉着。在较高压力（>500 Kpa）下，视网膜厚度增加，视网膜神经节细胞（RGC）层结构紊乱，RGC凋亡。伤眼眼压在伤后第1天和第7天明显下降（P < 0.05）。结论：利用自行研制的设备建立的兔眼闭合性冲击伤动物模型，能够成功模拟闭合性眼冲击伤的临床特点，且操作简单、可行。为今后研究闭合性地球仪冲击伤的机制和新的治疗措施提供了一种新的动物模型。
To establish a rabbit animal model of closed globe blast injury with an application of self-developed explosive injury equipment, we tend to explore the anatomic and pathological changes of eyes under different gas pressure. The device comprises of high-pressure air source compression pump, air channel, and gas shock. There were 36 healthy bluish blue rabbits exposed to one of five blast pressures (500, 1,000, 1,500, 2,000, and 5,000 Kpa). Slit lamp microscope, B-mode ultrasonography, fundus photography, optical coherence tomography (OCT), and intraocular pressure (IOP) examination were performed at 0-, 1-, 3-, and 7-days post exposure, while gross histopathology was assessed with H&E stain at 7 days. The contralateral eyes and non-blast exposed rabbits were used as controls. Definitive evidence of closed globe blast injury was obtained. Corneal edema and hyphema were observed in the models under all pressures with no full-thickness globe injury, or lens rupture, as the severity was pressure independent. There was no obvious retinal abnormality on B ultrasound or OCT scan, while light vitreous hemorrhage, commotio retinae, and heavy retinal pigmentation presented on one eye, respectively, in the eyes exposed to 5,000 Kpa. Increased retinal thickness with disorganizations on the retinal ganglion cell (RGC) layer and RGC apoptosis in groups under higher pressure (>500 Kpa). IOP of injured eyes were statistically decreased at day 1 and 7 post injury (p < 0.05). Conclusively, the rabbit animal model induced by self-developed equipment could mimic the clinical features of closed ocular blast injury successfully that was feasible and easy to operate. This will be a new rabbit animal model for investigating mechanisms and new therapeutic interventions of closed globe blast injury in the future.
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影响因子：
4.4
作者：
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通讯作者：
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