An assay for human telomeric G-quadruplex DNA binding drugs.
An assay for human telomeric G-quadruplex DNA binding drugs.
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DOI：
10.1016/j.bmcl.2013.10.030
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发表时间：
2013-12-15
影响因子：
2.7
通讯作者：
Arya, Dev P.
中科院分区：
文献类型：
--
作者：
Watkins, Derrick;Ranjan, Nihar;Kumar, Sunil;Gong, Changjun;Arya, Dev P.
Compounds that stabilize the G-quadruplexes formed by human telomeres can inhibit the telomerase activity and are potential cancer therapies. We have developed an assay for the screening of compounds with high affinity for human telomeric G-quadruplexes (HTG). The assay uses a thiazole orange fluorescent reporter molecule conjugated to the aminoglycoside, neomycin, as a probe in a fluorescence displacement assay. The conjugation of the planar base stacking thiazole orange with the groove binding neomycin results in high affinity probe that can determine the relative binding affinity of high affinity HTG binding drugs in a high throughput format. The robust assay is applicable for the determination of the binding affinity of HTG in the presence of K+ or Na+.
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影响因子：
1.3
作者：
Monchaud, D.;Allain, C.;Teulade-Fichou, M. -P
通讯作者：
Teulade-Fichou, M. -P
影响因子：
14.9
作者：
Ambrus A;Chen D;Dai J;Bialis T;Jones RA;Yang D
通讯作者：
Yang D
影响因子：
2.9
作者：
Xi, Hongjuan;Davis, Erik;Ranjan, Nihar;Xue, Liang;Hyde-Volpe, David;Arya, Dev P.
通讯作者：
Arya, Dev P.
影响因子：
5.7
作者：
WANG, Y;PATEL, DJ
通讯作者：
PATEL, DJ
影响因子：
2.9
作者：
Ranjan, Nihar;Andreasen, Katrine F.;Kumar, Sunil;Hyde-Volpe, David;Arya, Dev P.
通讯作者：
Arya, Dev P.