Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus.
Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus.
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DOI：
10.1016/j.jviromet.2015.04.008
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发表时间：
2015-08
影响因子：
3.1
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Yuan W;Li Y;Li P;Song Q;Li L;Sun J
建立了检测PEDV的nanoPCR方法。nanoPCR检测的灵敏度是常规RT-PCR检测的100倍。检测下限为2.7 × 10−6 ng/μL PEDV RNA。本试验可用于PEDV的临床诊断和现场监测。猪流行性腹泻病毒（Porcine epidemic diarrhea virus，PEDV）是一种引起猪呕吐、腹泻和脱水的重要病原体，对世界范围内的养猪业造成严重危害。在这项研究中，纳米颗粒辅助聚合酶链反应（nanoPCR）靶向PEDV的N基因的检测方法的灵敏度和特异性进行了研究。在用于检测PEDV RNA的优化条件下，nanoPCR测定比常规RT-PCR测定灵敏100倍。nanoPCR检测的检测下限为2.7 × 10−6 ng/μL PEDV RNA，未观察到与其他病毒的交叉反应。这是第一份证明应用nanoPCR检测PEDV的报告。本研究建立的灵敏度高、特异性强的nanoPCR检测方法可广泛应用于PEDV感染的临床诊断和现场监测。
A nanoPCR method was developed for the detection of PEDV. The nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit was 2.7 × 10−6 ng/μL of PEDV RNA. This test could be applied for clinical diagnosis and field surveillance of PEDV. Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was developed and the sensitivity and specificity were investigated. Under the optimized conditions for detection of PEDV RNA, the nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit of the nanoPCR assay was 2.7 × 10−6 ng/μL of PEDV RNA and no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of PEDV. The sensitive and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of PEDV-infection.
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影响因子：
3.1
作者：
Ma, Xingjie;Cui, Yuchao;Cui, Shangjin
通讯作者：
Cui, Shangjin
影响因子：
11.8
作者：
Puranaveja S;Poolperm P;Lertwatcharasarakul P;Kesdaengsakonwut S;Boonsoongnern A;Urairong K;Kitikoon P;Choojai P;Kedkovid R;Teankum K;Thanawongnuwech R
通讯作者：
Thanawongnuwech R
影响因子：
3.1
作者：
Li, Qiang;Zhou, Qing-feng;Cao, Yong-chang
通讯作者：
Cao, Yong-chang
一种检测猪博卡病毒的新型 nanoPCR 分子检测方法
影响因子：
3.1
作者：
Wang, Xiaoling;Bai, Aiquan;Cui, Shangjin
通讯作者：
Cui, Shangjin
影响因子：
3.3
作者：
Hou XL;Yu LY;Liu J
通讯作者：
Liu J