A kininogenase resembling glandular kallikrein in the rat pituitary pars intermedia.
A kininogenase resembling glandular kallikrein in the rat pituitary pars intermedia.
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一种类似于大鼠垂体中间部中腺体激肽释放酶的激肽原酶。
DOI：
10.1210/endo-112-4-1194
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
A. Nasjletti
中科院分区：
文献类型：
--
作者：
C. Powers;A. Nasjletti
本研究的目的是确定激肽生成蛋白酶（激肽原酶）在大鼠垂体组织中的分布和特征。将雄性大鼠垂体切成不同的叶，在含12 mM脱氧胆酸的生理盐水中超声，用激肽原和显色肽底物测定pH为8.5时的蛋白酶活性；用RIA法测定激肽的生成。发现大鼠垂体中含有高浓度的激肽原酶活性；该活性受到抑肽酶的强烈抑制，对大豆胰蛋白酶抑制剂具有抗性。大鼠尿钾激肽抗血清对垂体激肽原中间酶也有抑制作用。通过反相高效液相色谱法鉴定其产物为缓激肽。神经中间体垂体在0.25 M蔗糖缓冲液（pH 7.5）中匀浆，然后差速离心（1,000 X g， 5分钟；10,000 X g， 20分钟；105,000 X g， 70分钟），结果表明，大部分激肽原酶活性在10,000 X g颗粒中。垂体神经中间体提取物生成激肽的最适pH值为8.0，该提取物还可水解显色肽底物生成激肽。此外，神经中间体垂体提取物被发现含有一种独特的蛋白酶，可水解凝血酶的显色肽底物。垂体激肽原中间酶类似于腺体激肽素，可能参与激素原的加工。
The purpose of this study was to determine the distribution and characteristics of kinin-generating proteases (kininogenases) in rat pituitary tissue. Male rat pituitaries were dissected into their various lobes, sonicated in saline containing 12 mM deoxycholic acid, and assayed for protease activity at pH 8.5 using kininogen and chromogenic peptide substrates; kinin generation was measured by RIA. The rat pituitary was found to contain kininogenase activity highly concentrated in the pars intermedia; this activity was strongly inhibited by aprotinin and was resistant to soybean trypsin inhibitor. Antiserum against rat urinary kallikrein also inhibited the intermediate pituitary kininogenase. The kininogenase product was identified as bradykinin by reversed-phase high performance liquid chromatography. Homogenization of neurointermediate pituitaries in 0.25 M sucrose buffer (pH 7.5) followed by differential centrifugation (1,000 X g, 5 min; 10,000 X g, 20 min; 105,000 X g, 70 min) demonstrated that most of the kininogenase activity was in the 10,000 X g pellet. Kinin generation by neurointermediate pituitary extracts had a pH optimum of 8.0, and such extracts also hydrolyzed chromogenic peptide substrates for kallikreins. In addition, neurointermediate pituitary extracts were found to contain a distinct protease which hydrolyzed a chromogenic peptide substrate for thrombin. The intermediate pituitary kininogenase resembles glandular kallikrein and possibly may participate in prohormone processing.
不同物种垂体和脑组织中阿片黑皮质素原 mRNA 的检测和定量。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Civelli,O;Birnberg,N;Herbert,E
通讯作者：
Herbert,E
DOI：
10.1016/0006-291x(81)91823-4
发表时间：
1981-08
影响因子：
3.1
作者：
Narian Orlowski;Sherwin Wilk
通讯作者：
Narian Orlowski;Sherwin Wilk
猪垂体前叶中一种新型的激肽生成蛋白酶（激肽原酶）。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Powers,CA;Nasjletti,A
通讯作者：
Nasjletti,A