Chemical probing of glycans in cells and organisms.

Chemical probing of glycans in cells and organisms.
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DOI:
10.1039/c2cs35416k
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发表时间:
2013-05-21
影响因子:
46.2
通讯作者:
Wu P
Wu P
中科院分区:
化学1区
文献类型:
--
作者:
Rouhanifard SH;Nordstrøm LU;Zheng T;Wu P

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在生命的四个主要组成部分中,聚糖在许多生理和病理过程中发挥着重要作用。由于它们的非模板生物合成,与蛋白质和核酸研究的步伐相比,阐明聚糖功能的分子细节的进展相对缓慢。在过去的30年里,化学工具已经成为遗传学和分子生物学在天然环境中研究聚糖的强大盟友。本教程的审查将提供该领域的最新技术发展的概述,以及这些技术在细胞和生物体中探测聚糖的应用进展。
Among the four major building blocks of life, glycans play essential roles in numerous physiological and pathological processes. Due to their non-templated biosynthesis, advances towards elucidating the molecular details of glycan functions are relatively slow compared with the pace of protein and nucleic acid research. Over the past 30 years, chemical tools have emerged as powerful allies to genetics and molecular biology in the study of glycans in their native environment. This tutorial review will provide an overview of the recent technological developments in the field, as well as the progress in the application of these techniques to probe glycans in cells and organisms.
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