A histochemical study of the regeneration process after injury by pulsed Nd:YAG laser irradiation of root canals.
A histochemical study of the regeneration process after injury by pulsed Nd:YAG laser irradiation of root canals.
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脉冲 Nd:YAG 激光照射根管损伤后再生过程的组织化学研究。
DOI：
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发表时间：
2002
影响因子：
2.5
通讯作者：
Koukichi Matsumoto
中科院分区：
文献类型：
--
作者：
Y. Murakami;A. Unno;Y. Kimura;T. Okano;Mozammal Hossain;Yukio Nakamura;Koukichi Matsumoto
观察大鼠牙髓细胞损伤后的组织学变化。监测3、6和10天后的再生过程。用脉冲Nd：YAG激光以2 W和20脉冲每秒（pps）照射下颌切牙5秒，并对牙髓进行组织学和免疫化学检查TGF-β 1。发育中的牙齿的萌出仅在短时间内受到干扰。观察到骨牙本质的快速形成。3d后，可见纤维牙本质基质区及新生血管，随后可见再生牙本质形成，并在损伤区形成一层成牙本质细胞样细胞。TGF-β 1免疫组化染色显示，在套牙本质下的小组织区、纤维牙本质基质区和成牙本质样细胞中呈阳性表达。这些结果表明，脉冲Nd：YAG激光照射大鼠切牙牙髓诱导骨牙本质的形成，和TGF-β 1在再生过程中发挥作用。
Histological changes of rat dental pulp cells were followed after injury. The regeneration process after 3, 6 and 10 days was monitored. Mandibular incisors were irradiated with a pulsed Nd:YAG laser at 2 W and 20 pulses per sec (pps) for 5 sec and the pulp was examined histologically and immunohistochemically for TGF-beta1. Eruption of the developing tooth was disturbed for a short period only. Rapid formation of osteodentin was observed. After 3 days, a zone of fibrodentin matrix as well as newly formed vessels were found. Afterwards, regenerative dentin formation was observed accompanied by the formation of a layer of odontoblast-like cells in the damaged area. Immunohistochemical staining of TGF-beta1 showed that positivity was present in small tissue areas beneath the mantle dentin, the zone of fibrodentin matrix and odontoblast-like cells. These results indicate that pulsed Nd:YAG laser irradiation of rat incisor pulp induces formation of osteodentin, and TGF-beta1 plays a role during regeneration.
影响因子：
1.9
作者：
D'Souza, RN;Cavender, A;Letterio, J
通讯作者：
Letterio, J