Staphylococcus aureus extracellular vesicles (EVs): surface-binding antagonists of biofilm formation.
Staphylococcus aureus extracellular vesicles (EVs): surface-binding antagonists of biofilm formation.
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DOI：
10.1039/c7mb00365j
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发表时间：
2017-11-21
影响因子：
--
通讯作者：
Mitchell RJ
中科院分区：
文献类型：
--
作者：
Im H;Lee S;Soper SA;Mitchell RJ
金黄色葡萄球菌作为一种医院感染剂在世界范围内的流行已被公认，但这种细菌传播背后的原因仍然难以捉摸。在这里，我们假设金黄色葡萄球菌的传播可能受益于它阻止其他细菌在表面建立自己。几种病原体的情况就是如此，因为金黄色葡萄球菌上清液抑制了它们形成生物膜的能力。随后以鲍曼不动杆菌为模型的分析发现，这种效应主要是由金黄色葡萄球菌的胞外小泡(EVS)介导的，这些小泡结合在聚苯乙烯表面。我们发现，在EV处理后，EV处理的表面明显更亲水，这种情况使得鲍曼不动杆菌最初很难附着在聚苯乙烯表面，并使其产生的生物膜减少了93%。随后的测试发现，这也延伸到了其他几种细菌病原体，它们的生物被膜质量减少了40%-70%。用10%漂白剂洗涤表面后，金黄色葡萄球菌EVS及其活性仍保持不变，而乙二胺四乙酸(EDTA)去除了表面EVS及其活性。
The prevalence of Staphylococcus aureus worldwide as a nosocomial infectious agent is recognized but the reason behind the spread of this bacterium has remained elusive. Here, we hypothesized that the communication of S. aureus might benefit from it blocking other bacteria from establishing themselves on the surface. This was found to be the case for several pathogens as the S. aureus supernatant curtailed their ability to form biofilms. Subsequent analyses using Acinetobacter baumannii as a model found this effect is primarily mediated by S. aureus’ extracellular vesicles (EVs), which bound to the polystyrene surface. We found the EV-treated surfaces were significantly more hydrophilic after EV treatment, a condition that made it difficult for A. baumannii to initially adhere to the polystyrene surface and reduced its resulting biofilm by up to 93%. Subsequent tests found this also extended to several other bacterial pathogens, with a 40-70% decrease in their biofilm mass. The S. aureus EVs and their activity still remained after the surface was washed with 10% bleach, while the use of ethylenediaminetetraacetic acid (EDTA) removed both the EVs from the surface and their activity.
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DOI：
10.1038/nrmicro3480
发表时间：
2015-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Brown L;Wolf JM;Prados-Rosales R;Casadevall A
通讯作者：
Casadevall A
影响因子：
4.6
作者：
Brackman G;Breyne K;De Rycke R;Vermote A;Van Nieuwerburgh F;Meyer E;Van Calenbergh S;Coenye T
通讯作者：
Coenye T
影响因子：
14.9
作者：
Chabelskaya S;Bordeau V;Felden B
通讯作者：
Felden B
影响因子：
11.8
作者：
Begier, EM;Frenette, K;Hadler, JL
通讯作者：
Hadler, JL
影响因子：
6.9
作者：
Cheng, V. C. C.;Chau, P. H.;Yuen, K. Y.
通讯作者：
Yuen, K. Y.