Intercalation of herbicide propyzamide into DNA using acridine orange as a fluorescence probe
Intercalation of herbicide propyzamide into DNA using acridine orange as a fluorescence probe
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使用吖啶橙作为荧光探针将除草剂丙吡酰胺嵌入 DNA
DOI：
10.1016/j.snb.2014.09.114
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Xiaoyue Zhou
中科院分区：
文献类型：
--
作者：
Yue Zhang;Junhui Pan;Guowen Zhang;Xiaoyue Zhou
使用多光谱方法结合平行因子分析 (PARAFAC) 和分子对接确定 PRO 与小牛胸腺 DNA (ctDNA) 的结合作用。荧光滴定表明 PRO 的静态荧光猝灭是由 ctDNA 诱导的。焓变和熵变的正值表明结合反应主要由疏水相互作用驱动。 ctDNA 熔解温度和粘度的增加以及碘猝灭效应的降低表明 PRO 与 ctDNA 发生了嵌入结合。此外，通过PARAFAC模型解析了PRO和荧光探针吖啶橙（AO）与ctDNA之间竞争相互作用的三向同步荧光光谱数据，为反应体系提供了三种组分（PRO、AO和ctDNA-AO复合物）的浓度信息和光谱，这进一步支持了通过替换结合的AO探针将PRO分子嵌入ctDNA双螺旋中。分子对接预测ctDNA的A-T碱基对是PRO的主要结合位点，并通过傅里叶变换红外分析证实了这一点，并且从圆二色光谱研究中推断出PRO诱导的ctDNA从B型到A型的构象变化。
The binding action of PRO associated with calf thymus DNA (ctDNA) was determined using multispectroscopic methods combined with parallel factor analysis (PARAFAC) and molecular docking. The fluorescence titration suggested a static fluorescence quenching of PRO was induced by ctDNA. The positive values of enthalpy changes and entropy changes suggested that the binding reaction was predominantly driven by hydrophobic interactions. Increases in melting temperature and viscosity of ctDNA and decrease in iodide-quenching effect indicated that an intercalative binding of PRO to ctDNA occurred. Moreover, the three-way synchronous fluorescence spectra data from the competitive interaction between PRO and fluorescence probe acridine orange (AO) with ctDNA were resolved by PARAFAC modeling to provide the concentration information and spectra of the three components (PRO, AO and ctDNA–AO complex) for the reaction system, which further supported the intercalation of PRO molecule into ctDNA double-helix by replacing bound AO probe. The molecular docking predicted that the A–T base pairs of ctDNA were the main binding sites for PRO, which was confirmed by Fourier transform infrared analysis, and the conformational change of ctDNA from B-form to A-form induced by PRO was inferred from the circular dichroism spectra studies.
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DOI：
10.1016/j.saa.2012.05.045
发表时间：
2012-10
期刊：
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
影响因子：
--
作者：
N. Shahabadi;Saba Hadidi
通讯作者：
N. Shahabadi;Saba Hadidi
影响因子：
3.5
作者：
I. Ott;Yufang Xu;Jianwen Liu;M. Kokoschka;M. Harlos;W. Sheldrick;X. Qian
通讯作者：
I. Ott;Yufang Xu;Jianwen Liu;M. Kokoschka;M. Harlos;W. Sheldrick;X. Qian
影响因子：
11.2
作者：
M. Esteller;M. Esteller;P. Corn;S. Baylin;J. Herman
通讯作者：
M. Esteller;M. Esteller;P. Corn;S. Baylin;J. Herman
影响因子：
8.4
作者：
Dogan-Topal, Burcu;Bozal-Palabiyik, Burcin;Uslu, Bengi
通讯作者：
Uslu, Bengi
影响因子：
2.9
作者：
Andrushchenko, V;Leonenko, Z;Wieser, H
通讯作者：
Wieser, H