The DOCK protein sponge binds to ELMO and functions in Drosophila embryonic CNS development.
The DOCK protein sponge binds to ELMO and functions in Drosophila embryonic CNS development.
复制标题
DOI：
10.1371/journal.pone.0016120
复制
发表时间：
2011-01-25
期刊：
影响因子：
3.7
通讯作者：
Geisbrecht ER
中科院分区：
文献类型：
--
作者：
Biersmith B;Liu ZC;Bauman K;Geisbrecht ER
Cell morphogenesis, which requires rearrangement of the actin cytoskeleton, is essential to coordinate the development of tissues such as the musculature and nervous system during normal embryonic development. One class of signaling proteins that regulate actin cytoskeletal rearrangement is the evolutionarily conserved CDM (C. elegans Ced-5, human DOCK180, Drosophila Myoblast city, or Mbc) family of proteins, which function as unconventional guanine nucleotide exchange factors for the small GTPase Rac. This CDM-Rac protein complex is sufficient for Rac activation, but is enhanced upon the association of CDM proteins with the ELMO/Ced-12 family of proteins. We identified and characterized the role of Drosophila Sponge (Spg), the vertebrate DOCK3/DOCK4 counterpart as an ELMO-interacting protein. Our analysis shows Spg mRNA and protein is expressed in the visceral musculature and developing nervous system, suggesting a role for Spg in later embryogenesis. As maternal null mutants of spg die early in development, we utilized genetic interaction analysis to uncover the role of Spg in central nervous system (CNS) development. Consistent with its role in ELMO-dependent pathways, we found genetic interactions with spg and elmo mutants exhibited aberrant axonal defects. In addition, our data suggests Ncad may be responsible for recruiting Spg to the membrane, possibly in CNS development. Our findings not only characterize the role of a new DOCK family member, but help to further understand the role of signaling downstream of N-cadherin in neuronal development.
登录
查看更多内容
DOI：
10.1523/jneurosci.3985-08.2009
发表时间：
2009-01-07
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Chen Q;Peto CA;Shelton GD;Mizisin A;Sawchenko PE;Schubert D
通讯作者：
Schubert D
影响因子：
21.3
作者：
Côté, JF;Motoyama, AB;Vuori, K
通讯作者：
Vuori, K
影响因子：
1.6
作者：
Geisbrecht, BV;Bouyain, S;Pop, M
通讯作者：
Pop, M
影响因子：
4.6
作者：
Forsthoefel, DJ;Liebl, EC;Seeger, MA
通讯作者：
Seeger, MA
影响因子：
64.8
作者：
Hakeda-Suzuki, S;Ng, J;Dickson, BJ
通讯作者：
Dickson, BJ