Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model.
Integrative Analysis of miRNA and mRNA Expression Profiles in Calcium Oxalate Nephrolithiasis Rat Model.
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草酸钙肾结石大鼠模型中 miRNA 和 mRNA 表达谱的综合分析
DOI：
10.1155/2017/8306736
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发表时间：
2017
影响因子：
--
通讯作者：
Wu W
中科院分区：
文献类型：
--
作者：
Lan C;Chen D;Liang X;Huang J;Zeng T;Duan X;Chen K;Lai Y;Yang D;Li S;Jiang C;Wu W
微RNA(MiRNA)在草酸钙肾结石中的表达谱及其生物学功能尚不清楚。在本研究中，我们研究了草酸钙结石大鼠肾脏组织中miRNA和mRNA的表达谱。SD大鼠16只，随机分为对照组和结石形成组。4周后采集24小时尿样和肾组织进行生化和组织学检查。应用miRNA和mRNA微阵列技术检测miRNA和mRNAs的表达。为了验证基因芯片的结果，进行了实时定量聚合酶链式反应(qRT-PCR)。有38个miRNAs和2728个mRNAs在成石组和对照组肾组织中有显著差异表达。基因本体论(GO)分析表明，大多数靶基因在氧化还原、离子转运、炎症反应和创伤反应方面都得到了丰富的表达。京都基因和基因组百科全书(KEGG)对这些靶标的通路分析强调了它们在细胞因子-细胞因子受体相互作用、缝隙连接和趋化因子信号通路中的关键作用。此外，通过qRT-PCR检测，验证了基于微阵列的结果的可靠性。草酸钙结石大鼠肾脏miRNA和mRNA的表达可能为进一步研究尿石症的发病机制提供依据。
The microRNA (miRNA) expression profiles and their biological functions in calcium oxalate nephrolithiasis remain unclear. In this study, we investigate the miRNA and mRNA expression profiles of kidney tissues in calcium oxalate stone rats. 16 Sprague Dawley rats were divided into control group and stone-forming group. 24-hour urine samples and kidney tissues were collected for biochemical and histological determination after 4 weeks. MiRNA and mRNA microarray were applied to evaluate the miRNA and mRNA expression profiles. To validate the microarray results, the quantitative real-time PCR (qRT-PCR) was performed. A total of 38 miRNAs and 2728 mRNAs were significantly and differentially expressed in kidney tissues of stone-forming group versus control group. Gene Ontology (GO) analysis revealed that most of the target genes were enriched in terms of oxidation reduction, ion transport, inflammatory response, and response to wounding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of these targets highlights their critical role in cytokine-cytokine receptor interaction, gap junction, and chemokine signaling pathway. Furthermore, the reliability of the microarray-based results was confirmed by using qRT-PCR determination. The miRNA and mRNA expressions in calcium oxalate stone rat kidneys might provide a basis for further research on urolithiasis mechanism.
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影响因子：
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作者：
Casaburi I;Cesario MG;Donà A;Rizza P;Aquila S;Avena P;Lanzino M;Pellegrino M;Vivacqua A;Tucci P;Morelli C;Andò S;Sisci D
通讯作者：
Sisci D
DOI：
10.1016/j.juro.2015.11.048
发表时间：
2016-04
期刊：
The Journal of urology
影响因子：
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作者：
Kusmartsev S;Dominguez-Gutierrez PR;Canales BK;Bird VG;Vieweg J;Khan SR
通讯作者：
Khan SR
肾结石患者血液和尿液中 miR-155 水平升高
影响因子：
--
作者：
Hu YY;Dong WD;Xu YF;Yao XD;Peng B;Liu M;Zheng JH
通讯作者：
Zheng JH
影响因子：
3.2
作者：
Liu, Xiujuan;Hong, Quan;Xu, Lihong
通讯作者：
Xu, Lihong
影响因子：
--
作者：
Lin, Qinqin;Geng, Yuanwen;Tian, Zhenjun
通讯作者：
Tian, Zhenjun