Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.
Elevated levels of miR-155 in blood and urine from patients with nephrolithiasis.
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肾结石患者血液和尿液中 miR-155 水平升高
DOI：
10.1155/2014/295651
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发表时间：
2014
影响因子：
--
通讯作者：
Zheng JH
中科院分区：
文献类型：
--
作者：
Hu YY;Dong WD;Xu YF;Yao XD;Peng B;Liu M;Zheng JH
背景循环和尿液中的miRNAs可能是一种潜在的非侵入性分子生物标志物，能够预测慢性肾脏疾病，在本研究中，我们将研究肾结石患者血清和尿液中miR-155的水平。方法.在60名肾结石患者中定量miR-155的血清和尿液水平;将结果与50名健康志愿者进行比较。计算估计肾小球滤过率（eGFR），并通过简单回归分析，分析miR-155/eGFR和miR-155/CRP（C反应蛋白）水平的相关性。结果肾结石患者的血清和尿液中miR-155水平的中位数显著高于对照组。eGFR与尿miR-155水平呈负相关; CRP与尿miR-155呈正相关。miR-155的尿水平与白细胞介素-（IL-）1β、IL-6和肿瘤坏死因子-（TNF-）α的尿表达呈负相关，与活化后调节、正常T细胞表达和分泌（RANTES）的尿表达呈正相关。结论肾结石患者血清和尿液中miR-155水平显著升高，且miR-155的上调与eGFR下降和CRP升高相关。结果提示，miR-155可能通过调节炎症因子的表达，在肾结石的病理生理过程中发挥重要作用。需要进一步研究其分子致病机制并进行更大规模的临床试验。
Background. Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis. Methods. Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well. Results. The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES). Conclusion. Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.
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