The effects of terbium (III) on the Ca-activated, K channel found in the resealed human erythrocyte membrane.
The effects of terbium (III) on the Ca-activated, K channel found in the resealed human erythrocyte membrane.
复制标题
铽 (III) 对重新密封的人红细胞膜中 Ca 激活的 K 通道的影响。
DOI：
10.1111/j.1432-1033.1985.tb08620.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
H. Mueller
中科院分区：
文献类型：
--
作者：
P. Wood;H. Mueller
掺入的铽Tb3+激活了在重新密封的红细胞鬼膜中发现的Ca激活的K通道，并允许K的净流出。在Ca激活的情况下，低水平的外部K刺激K的净流出。掺入的Tb3+的作用似乎与掺入的Ca类似。然而，在Ca或Tb3+激活后，外部施用的Tb3+抑制了K的净流出。即使在低水平的合并胰蛋白酶部分消化通道后，Tb3+也能抑制K的净流出。此外，当通道掺入平面双层时，可以被Tb3+抑制。外部施用Tb3+似乎不会通过抑制共运输阴离子的途径间接抑制净K外排。此外，由于抑制所需的Tb3+浓度较低，表面电位的改变似乎不太可能是观察到的效果的原因。
Incorporated terbium, Tb3+, activates the Ca-activated K channel found in the resealed erythrocyte ghost membrane and allows the net efflux of K. As in the case of Ca activation, low levels of external K stimulate the net efflux of K. The action of incorporated Tb3+ seems to be analogous to that of incorporated Ca. Externally applied Tb3+, however, inhibits the net efflux of K after either Ca or Tb3+ activation. The net-efflux of K can be inhibited by Tb3+ even after partial digestion of the channel by low levels of incorporated trypsin. Furthermore, the channel when incorporated into planar bilayers, can be inhibited by Tb3+. Externally applied Tb3+ does not seem to inhibit net K efflux indirectly via inhibition of the pathways for the co-transported anions. In addition because of the low concentrations of Tb3+ required for inhibition, it seems unlikely that an alteration of surface potential is responsible for the observed effects.
DOI：
10.1073/pnas.81.9.2772
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MILLER, C;WHITE, MM
通讯作者：
WHITE, MM