Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking.

Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking.
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DOI:
10.1002/anie.201108695
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发表时间:
2012-05-07
影响因子:
16.6
通讯作者:
Chiu, Daniel T.
Chiu, Daniel T.
中科院分区:
化学1区
文献类型:
--
作者:
Schiro, Perry G.;Zhao, Mengxia;Kuo, Jason S.;Koehler, Karen M.;Sabath, Daniel E.;Chiu, Daniel T.

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This paper describes an approach called ensemble decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood. eDAR has a recovery of over 93% (n=9) with a zero false positive rate (n=8), and provides direct easy access to individual isolated live cells for downstream single-cell manipulation and analysis. We anticipate eDAR will enable new studies of various types of rare cells that circulate in blood.
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