Increase of the expression and activity of ferredoxin-NADP+ oxidoreductase in the cells adapted to low CO2 in the cyanobacterium Synechocystis 6803
Increase of the expression and activity of ferredoxin-NADP+ oxidoreductase in the cells adapted to low CO2 in the cyanobacterium Synechocystis 6803
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蓝藻集胞藻 6803 适应低 CO2 的细胞中铁氧还蛋白-NADP 氧化还原酶的表达和活性增加
DOI：
10.1007/s11099-010-0054-z
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发表时间：
2010-10
期刊：
影响因子：
2.7
通讯作者：
Ma Weimin
中科院分区：
文献类型：
--
作者：
Mi Hualing;Liu Yanru;Ma Weimin
研究低CO2对铁氧还蛋白-NADP+氧化还原酶（FNR）的表达和活性以及该酶介导的光系统I周围循环电子流的影响（循环PSI），活性染色，在高CO2或低CO2生长（H或L）的集胞藻属野生型菌株PCC 6803（WT）及其ΔndhB突变体（M55）的细胞中测量免疫印迹和P700+还原的初始速率。.主要结果描述如下。(1)WT和M55的L细胞中FNR的蛋白水平和活性均显著高于H细胞。(2)与相应的H细胞相比，WT的L细胞的循环PSI率显著增加，而不是M55。(3)FNR抑制剂N-乙基马来酰亚胺部分抑制WT和M55中低CO2诱导的循环PSI速率的增加。这些结果表明，低CO2浓度下FNR的表达和活性增强，FNR介导的PSI循环也增强。FNR对环状PSI的贡献进行了简要讨论。
To investigate the effect of low CO2 on the expression and activity of ferredoxin-NADP+ oxidoreductase (FNR) and this enzyme-mediated cyclic electron flow around photosystem I (cyclic PSI), the activity staining, immunoblotting and initial rate of P700+ reduction were measured in high- or low-CO2-grown (H or L)-cells of wild-type Synechocystis sp. strain PCC 6803 (WT) and its ΔndhB mutant (M55). Major results were depicted as follows. (1) The protein levels and activity of FNR were remarkably stimulated in L-cells of both WT and M55 relative to that in their H-cells. (2) The rate of cyclic PSI was significantly increased in L-cells of WT, not M55, when compared to that in respective H-cells. (3) N-ethylmaleimide, an inhibitor of FNR, partially inhibited the increase in the rate of cyclic PSI induced by low CO2 in both WT and M55. These findings indicated that low CO2 enhanced the expression and activity of FNR and the cyclic PSI mediated by FNR. The contribution of FNR to cyclic PSI is shortly discussed.
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影响因子：
4.9
作者：
Weimin Ma;Yong Deng;T. Ogawa;H. Mi
通讯作者：
Weimin Ma;Yong Deng;T. Ogawa;H. Mi
影响因子：
3.7
作者：
SCHREIBER, U;SCHLIWA, U;BILGER, W
通讯作者：
BILGER, W
DOI：
10.1105/tpc.104.026526
发表时间：
2004-12
期刊：
The Plant Cell Online
影响因子：
--
作者：
Pengpeng Zhang;N. Battchikova;T. Jansén;Jens Appel;T. Ogawa;E. Aro
通讯作者：
Pengpeng Zhang;N. Battchikova;T. Jansén;Jens Appel;T. Ogawa;E. Aro
DOI：
10.1007/978-1-4615-4827-0
发表时间：
1999
期刊：
--
影响因子：
--
作者：
G. Peschek;W. Löffelhardt;G. Schmetterer
通讯作者：
G. Peschek;W. Löffelhardt;G. Schmetterer
DOI：
10.1073/pnas.191258298
发表时间：
2001-09-25
影响因子：
11.1
作者：
Shibata, M;Ohkawa, H;Ogawa, T
通讯作者：
Ogawa, T