Expression and clinical significance of multidrug resistance proteins in brain tumors.
Expression and clinical significance of multidrug resistance proteins in brain tumors.
复制标题
DOI：
10.1186/1756-9966-29-122
复制
发表时间：
2010-09-05
期刊：
影响因子：
--
通讯作者：
Jin X
中科院分区：
文献类型：
--
作者：
Guo Z;Zhu J;Zhao L;Luo Q;Jin X
目的：探讨脑肿瘤多药耐药的机制，定位P-gp在人脑组织中的细胞表达部位，并从形态上确定P-gp与小窝蛋白-1之间是否存在关联。应用免疫组织化学方法检测P-糖蛋白(P-gp)、多药耐药相关蛋白(Mdr)、肺耐药相关蛋白(Lrp)、拓扑异构酶II(TOPO II)和谷胱甘肽-S-π(Gst-π)在30例脑肿瘤组织和5例正常脑组织中的表达和定位。对P-gp(TRITC标记)和小窝蛋白-1(FITC标记)进行双重标记。用激光扫描显微镜观察这两种蛋白在血脑屏障中的定位和表达特征。P-gp在血管壁及血管周围组织中呈高表达。而P-gp在肿瘤细胞中的表达较低。其余4种多药耐药蛋白在血管壁均未见表达。激光扫描显微镜显示P-gp和小窝蛋白-1共表达：这两种蛋白共定位于腔内皮室或腔/腔室交界处。化疗药物被P-gp阻断在血脑屏障神经上皮细胞的末端，从而削弱了化疗效果。P-gp标记血脑屏障，转运蛋白定位于腔内皮细胞间，与小窝蛋白-1共定位。
To investigate the mechanisms of multidrug resistance of brain tumors, to identify the site of cellular expression of P-gp in human brains in situ and to morphologically determine whether an association may exist between P-gp and caveolin-1. Immunohistochemistry was used to detect the expression and location of P-glycoprotein (P-gp), Multidrug resistance-associated protein (MDR), Lung resistance-related protein (LRP), Topoisomerase II (Topo II) and Glutathione-S-π (GST-π) in 30 patient tumor tissues and 5 normal brain tissues. The sections were subjected to double labeling for P-gp (TRITC labeled) and caveolin-1 (FITC labeled). The location and characteristics of expression of the two proteins in the blood brain barrier(BBB) was observed using a laser scanning microscope. High expression of P-gp was detected in vessel walls and the tissue surrounding the vessels. However, expression of P-gp was low in tumor cells. The expression of the other 4 multidrug resistance proteins was not observed in the vessel walls. Laser scanning microscopy showed P-gp and caveolin-1 co-expression: the two proteins co-localized either in the luminal endothelial compartment or at the border of the luminal/abluminal compartments. Chemotherapeutics drugs are interrupted in the end-feet of neuroepithelial cells of the BBB by P-gp, which weakens the chemotherapeutic effect. P-gp marks the BBB, and the transporter is localized in the luminal endothelial compartment where it co-localizes with caveolin-1.
登录
查看更多内容
影响因子：
3.2
作者：
Virgintino, D;Robertson, D;Bertossi, M
通讯作者：
Bertossi, M
DOI：
10.1073/pnas.92.22.10104
发表时间：
1995-10-24
影响因子：
11.1
作者：
SMART, EJ;YING, YS;ANDERSON, RGW
通讯作者：
ANDERSON, RGW
影响因子：
3
作者：
Lavie, Y;Liscovitch, M
通讯作者：
Liscovitch, M
DOI：
10.1006/bbrc.2001.4312
发表时间：
2001-03-02
影响因子：
3.1
作者：
Demeule, M;Labelle, M;Béliveau, R
通讯作者：
Béliveau, R
影响因子：
2.9
作者：
Golden, PL;Pardridge, WM
通讯作者：
Pardridge, WM