Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins
Identification of a domain of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation through the use of protein A fusion proteins
复制标题
通过使用蛋白 A 融合蛋白鉴定蛋白-DNA 复合物形成所需的单纯疱疹病毒反式激活因子 Vmw65 的结构域
作者：
G. Werstuck;J. Capone
In order to identify structural domains of the herpes simplex virus trans-activator Vmw65 required for protein-DNA complex formation, subfragments of Vmw65 were expressed in Escherichia coli as fusion polypeptides with protein A of Staphylococcus aureus, and the purified hybrids were used in a band shift assay. The results indicate that a region near the amino terminus of Vmw65 between amino acids 141 and 185 is necessary for complex formation.
DOI：
10.1073/pnas.84.1.71
发表时间：
1987-01-01
影响因子：
11.1
作者：
KRISTIE, TM;ROIZMAN, B
通讯作者：
ROIZMAN, B