Chromatin compaction protects genomic DNA from radiation damage.
Chromatin compaction protects genomic DNA from radiation damage.
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DOI：
10.1371/journal.pone.0075622
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Maeshima K
中科院分区：
文献类型：
--
作者：
Takata H;Hanafusa T;Mori T;Shimura M;Iida Y;Ishikawa K;Yoshikawa K;Yoshikawa Y;Maeshima K
基因组DNA在细胞核中以三维方式组织，被认为形成了紧密的染色质结构域。尽管已知染色质紧凑对有丝分裂是必不可少的，但它是否具有其他优势，特别是在间期细胞中，目前尚不清楚。在这里，我们报告了染色质紧致保护基因组DNA免受辐射损伤。利用一种新开发的固相体系，我们发现电离辐射后紧密染色质的双链断裂(DSB)频率比去浓缩染色质低5-50倍。由于自由基清除剂抑制了去缩染色质中DSB的诱导，浓缩染色质在电离辐射后具有较低的反应性自由基产生水平。我们还发现，染色质的紧致作用可以保护DNA免受化学试剂的攻击。我们的发现表明，基因组DNA紧凑在维持基因组完整性方面起着重要作用。
Genomic DNA is organized three-dimensionally in the nucleus, and is thought to form compact chromatin domains. Although chromatin compaction is known to be essential for mitosis, whether it confers other advantages, particularly in interphase cells, remains unknown. Here, we report that chromatin compaction protects genomic DNA from radiation damage. Using a newly developed solid-phase system, we found that the frequency of double-strand breaks (DSBs) in compact chromatin after ionizing irradiation was 5–50-fold lower than in decondensed chromatin. Since radical scavengers inhibited DSB induction in decondensed chromatin, condensed chromatin had a lower level of reactive radical generation after ionizing irradiation. We also found that chromatin compaction protects DNA from attack by chemical agents. Our findings suggest that genomic DNA compaction plays an important role in maintaining genomic integrity.
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影响因子：
5.3
作者：
Bryant, Jessica M.;Govin, Jerome;Berger, Shelley L.
通讯作者：
Berger, Shelley L.
DOI：
10.1073/pnas.0810057105
发表时间：
2008-12-16
影响因子：
11.1
作者：
Eltsov, Mikhail;MacLellan, Kirsty M.;Dubochet, Jacques
通讯作者：
Dubochet, Jacques
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
影响因子：
64.8
作者：
Dixon, Jesse R.;Selvaraj, Siddarth;Yue, Feng;Kim, Audrey;Li, Yan;Shen, Yin;Hu, Ming;Liu, Jun S.;Ren, Bing
通讯作者：
Ren, Bing
影响因子：
3.7
作者：
Cowell, Ian G.;Sunter, Nicola J.;Singh, Prim B.;Austin, Caroline A.;Durkacz, Barbara W.;Tilby, Michael J.
通讯作者：
Tilby, Michael J.