Differential ability of proximal and remote element pairs to cooperate in activating RNA polymerase II transcription
Differential ability of proximal and remote element pairs to cooperate in activating RNA polymerase II transcription
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近端和远端元件对协同激活 RNA 聚合酶 II 转录的差异能力
DOI：
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发表时间：
1991
影响因子：
5.3
通讯作者：
J. Gralla
中科院分区：
文献类型：
--
作者：
W. Wang;J. Gralla
为了研究转录元件之间的协同作用或协同相互作用，我们设计并构建了一系列具有不同元件组合的合成聚合酶II启动子。其中包括三个不同的 CCAAT 盒（对应于 CP1、CP2 和 NFI 的结合位点）、GC 盒、CACCC 盒和 ATF/CREB 结合位点。在近端位置含有这些元件的合成启动子与测试基因（CAT）连接。 AP1 和 AP2 结合位点、猿猴病毒 40 增强子和 GAL 雌激素受体 DNA 结合位点的串联重复序列被克隆到测试基因的下游。然后在 HeLa TK 细胞中进行瞬时表达测定，测试这些启动子的强度。在腺病毒主要晚期启动子 TATA 盒的背景下，仅包含某些元件组合的启动子在此测定中具有活性。有些元素似乎几乎普遍合作，但另一些则表现出很强的选择性。这些结果表明元件之间存在强选择性协同相互作用，并表明启动子强度水平可能由与近端位点和增强子位点结合的因子之间的相容性程度决定。
To investigate the synergism or cooperative interaction between transcription elements, we have designed and constructed a series of synthetic polymerase II promoters with different combinations of elements. These include three different CCAAT boxes, which correspond to the binding sites for CP1, CP2, and NFI, a GC box, a CACCC box, and an ATF/CREB-binding site. The synthetic promoters containing these elements in proximal positions were linked to a test gene (CAT). Tandem repeats of AP1- and AP2-binding sites, the simian virus 40 enhancer, and DNA-binding sites for GAL-estrogen receptor were cloned downstream of the test gene. The strength of these promoters was then tested in transient-expression assays in HeLa TK- cells. In the context of the adenovirus major late promoter TATA box, the promoters containing only certain combinations of elements are active in this assay. Some elements appear to cooperate nearly universally, but others exhibit strong selectivity. These results indicate strongly selective synergistic interactions between elements and suggest that levels of promoter strength may be determined by the extent of compatibility between factors bound to proximal and enhancer sites.
上游调控区需要稳定与腺病毒早期启动子中 TATA 序列的结合。
影响因子：
14.9
作者：
Garcia,J;Wu,F;Gaynor,R
通讯作者：
Gaynor,R
巨细胞病毒基因表达的调节：α和β启动子在允许的人成纤维细胞中被病毒功能反式激活。
影响因子：
5.4
作者：
Spaete,RR;Mocarski,ES
通讯作者：
Mocarski,ES