DNA-SIP based genome-centric metagenomics identifies key long-chain fatty acid-degrading populations in anaerobic digesters with different feeding frequencies.
DNA-SIP based genome-centric metagenomics identifies key long-chain fatty acid-degrading populations in anaerobic digesters with different feeding frequencies.
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DOI：
10.1038/ismej.2017.143
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Beck DAC
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文献类型：
--
作者：
Ziels RM;Sousa DZ;Stensel HD;Beck DAC
脂肪、油和油脂（FOG）是高能量的废物，可以添加到厌氧消化器中，通过长链脂肪酸（LCFA）的转化，大大提高生物甲烷的回收率。然而，需要更好地了解厌氧消化池中互养LCFA降解微生物群落的生态生理学，以制定减轻FOG抑制LCFA积累的操作策略。在这项研究中，DNA稳定同位素探测（SIP）加上宏基因组测序的基因组为中心的比较油酸（C18：1）降解人口在两个厌氧codesters操作与脉冲进料或连续进料策略。脉冲进料的共消化器微宇宙以比连续进料的共消化器微宇宙高20%以上的速率将油酸转化成甲烷。首次证明了差异覆盖分箱从DNA-SIP宏基因组中恢复群体基因组箱（GB）。约70%的13 C-富集的GB被分类分配到互养单胞菌属，从而证实了互养单胞菌属物种在厌氧消化器中对LCFA降解的重要性。13 C-富集的GBs的系统发育比较表明，遗传学上不同的互养单胞菌GBs是独特的每个codigester。总体而言，这些结果表明，在厌氧消化池中的互养人口可以有不同的适应能力，并选择不同的人口可以通过调整反应器的操作条件，以最大限度地提高生物甲烷回收。
Fats, oils and greases (FOG) are energy-dense wastes that can be added to anaerobic digesters to substantially increase biomethane recovery via their conversion through long-chain fatty acids (LCFAs). However, a better understanding of the ecophysiology of syntrophic LCFA-degrading microbial communities in anaerobic digesters is needed to develop operating strategies that mitigate inhibitory LCFA accumulation from FOG. In this research, DNA stable isotope probing (SIP) was coupled with metagenomic sequencing for a genome-centric comparison of oleate (C18:1)-degrading populations in two anaerobic codigesters operated with either a pulse feeding or continuous-feeding strategy. The pulse-fed codigester microcosms converted oleate into methane at over 20% higher rates than the continuous-fed codigester microcosms. Differential coverage binning was demonstrated for the first time to recover population genome bins (GBs) from DNA-SIP metagenomes. About 70% of the 13C-enriched GBs were taxonomically assigned to the Syntrophomonas genus, thus substantiating the importance of Syntrophomonas species to LCFA degradation in anaerobic digesters. Phylogenetic comparisons of 13C-enriched GBs showed that phylogenetically distinct Syntrophomonas GBs were unique to each codigester. Overall, these results suggest that syntrophic populations in anaerobic digesters can have different adaptive capacities, and that selection for divergent populations may be achieved by adjusting reactor operating conditions to maximize biomethane recovery.
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影响因子：
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作者：
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10.1038/ismej.2015.37
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