Mapping Enzyme Landscapes by Time-Resolved Crystallography with Synchrotron and X-Ray Free Electron Laser Light.
Mapping Enzyme Landscapes by Time-Resolved Crystallography with Synchrotron and X-Ray Free Electron Laser Light.
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DOI：
10.1146/annurev-biophys-100421-110959
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发表时间：
2022-05-09
影响因子：
12.4
通讯作者：
中科院分区：
文献类型：
--
作者：
在分子水平上直接实时观察酶的催化作用一直是结构酶学的长期目标。同步加速器和X射线自由电子激光(XFEL)源上的时间分辨系列结晶学方法使人们能够在环境条件下以前所未有的时间分辨率跟踪酶催化和其他非平衡事件。X射线结晶学提供了有关构象异质性和蛋白质动力学的详细信息，当使用时间分辨方法时，这些信息会得到增强。本文概述了从X射线结晶学数据中提取蛋白质潜在能量谱信息的方法，重点介绍了XFEL和同步辐射时间分辨结晶学的新进展。由这些技术提供的酶催化的新兴观点可以被解释为酶在依赖于时间的能量格局上移动。讨论了这一观点的一些后果，包括不可逆酶或使用共价催化机制的酶通常可能表现出催化激活的运动的建议。
Directly observing enzyme catalysis in real-time at the molecular level has been a long-standing goal of structural enzymology. Time-resolved serial crystallography methods at synchrotron and X-ray free electron laser (XFEL) sources have enabled enzyme catalysis and other non-equilibrium events to be followed at ambient conditions with unprecedented time resolution. X-ray crystallography provides detailed information about conformational heterogeneity and protein dynamics, which is enhanced when time-resolved approaches are used. This review outlines the ways that information about the underlying energy landscape of a protein can be extracted from X-ray crystallographic data, with an emphasis on new developments in XFEL and synchrotron time-resolved crystallography. The emerging view of enzyme catalysis afforded by these techniques can be interpreted as enzymes moving on a time-dependent energy landscape. Some consequences of this view are discussed, including the proposal that irreversible enzymes or enzymes that use covalent catalytic mechanisms may commonly exhibit catalysis-activated motions.
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DOI：
10.1126/science.1217737
发表时间：
2012-07-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boutet S;Lomb L;Williams GJ;Barends TR;Aquila A;Doak RB;Weierstall U;DePonte DP;Steinbrener J;Shoeman RL;Messerschmidt M;Barty A;White TA;Kassemeyer S;Kirian RA;Seibert MM;Montanez PA;Kenney C;Herbst R;Hart P;Pines J;Haller G;Gruner SM;Philipp HT;Tate MW;Hromalik M;Koerner LJ;van Bakel N;Morse J;Ghonsalves W;Arnlund D;Bogan MJ;Caleman C;Fromme R;Hampton CY;Hunter MS;Johansson LC;Katona G;Kupitz C;Liang M;Martin AV;Nass K;Redecke L;Stellato F;Timneanu N;Wang D;Zatsepin NA;Schafer D;Defever J;Neutze R;Fromme P;Spence JC;Chapman HN;Schlichting I
通讯作者：
Schlichting I
影响因子：
56.9
作者：
Burling, FT;Weis, WI;Brunger, AT
通讯作者：
Brunger, AT
DOI：
10.1073/pnas.82.15.5000
发表时间：
1985-01-01
影响因子：
11.1
作者：
ANSARI, A;BERENDZEN, J;YOUNG, RD
通讯作者：
YOUNG, RD
影响因子：
5.7
作者：
Chen, Xiaorui;Poon, Billy K.;Ma, Jianpeng
通讯作者：
Ma, Jianpeng
DOI：
10.1073/pnas.0706443104
发表时间：
2007-11-20
影响因子：
11.1
作者：
Arora, Karunesh;Brooks, Charles L., III
通讯作者：
Brooks, Charles L., III