A genomic atlas of mouse hypothalamic development.
A genomic atlas of mouse hypothalamic development.
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DOI：
10.1038/nn.2545
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发表时间：
2010-06
影响因子：
25
通讯作者：
Blackshaw, Seth
中科院分区：
文献类型：
--
作者：
Shimogori, Tomomi;Lee, Daniel A.;Miranda-Angulo, Ana;Yang, Yanqin;Wang, Hong;Jiang, Lizhi;Yoshida, Aya C.;Kataoka, Ayane;Mashiko, Hiromi;Avetisyan, Marina;Qi, Lixin;Qian, Jiang;Blackshaw, Seth
下丘脑是许多对生存至关重要的行为的中央调节器，例如温度调节，食物摄入和昼夜节律。然而，介导下丘脑发育的分子途径在很大程度上是未知的。为了鉴定发育中小鼠下丘脑表达的基因，我们在12个不同的发育时间点进行了微阵列分析。然后，我们对下丘脑神经发生过程中动态表达的1,045个基因进行了发育原位杂交。我们确定了在整个神经发生过程中稳定标记每个主要下丘脑核团的标记物，并构建了发育中下丘脑的详细分子图谱。作为这些数据的实用性的概念证明，我们使用这些标记物来分析小鼠的表型，其中Sonic Hedgehog（Shh）被选择性地从下丘脑神经上皮中删除，并且发现Shh对于前下丘脑图案化是必需的。我们的研究结果作为下丘脑发育，连接，生理和功能障碍的功能研究的资源。
The hypothalamus is a central regulator of many behaviors that are essential for survival, such as temperature regulation, food intake and circadian rhythms. However, the molecular pathways that mediate hypothalamic development are largely unknown. To identify genes expressed in developing mouse hypothalamus, we performed microarray analysis at 12 different developmental time points. We then conducted developmental in situ hybridization for 1,045 genes that were dynamically expressed over the course of hypothalamic neurogenesis. We identified markers that stably labeled each major hypothalamic nucleus over the entire course of neurogenesis and constructed a detailed molecular atlas of the developing hypothalamus. As a proof of concept of the utility of these data, we used these markers to analyze the phenotype of mice in which Sonic Hedgehog (Shh) was selectively deleted from hypothalamic neuroepithelium and found that Shh is essential for anterior hypothalamic patterning. Our results serve as a resource for functional investigations of hypothalamic development, connectivity, physiology and dysfunction.
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