Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis.
Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis.
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DOI：
10.1186/s13568-015-0115-6
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ueda M
中科院分区：
文献类型：
--
作者：
Aburaya S;Esaka K;Morisaka H;Kuroda K;Ueda M
纤维素梭菌是一种厌氧纤维素分解细菌，能有效降解和代谢各种底物，包括纤维素、半纤维素(木聚糖和半乳甘露聚糖)和果胶。在梭状芽孢杆菌中，这种降解和代谢广泛的半纤维素和果胶底物的能力是一个独特的特征；然而，其机制目前尚不清楚。为了阐明半纤维素和果胶的识别和代谢机制，我们对在这些底物上培养的毛囊线虫进行了蛋白质组定量分析。用葡萄糖、木聚糖、半乳甘露聚糖(LBG)或果胶培养36小时，用木聚糖和半乳甘露聚糖寻找半纤维素的共同识别机制，用果胶寻找纤维素识别的独特系统。利用等压标记法和配备长整体硅胶毛细管柱的液-质联用仪，我们从所有底物中鉴定了734个胞内蛋白质。我们对所得到的蛋白质进行了KEGG分析和聚类分析。在KEGG分析中，我们发现了半纤维素和果胶的共同降解机制。在与基因组分析相对应的聚类分析中，我们检测到底物特异性簇，包括与底物识别、底物降解和代谢相关的基因。结合KEGG分析和聚类分析的结果，我们提出了半纤维素和果胶在纤维素假丝酵母中的识别和代谢机制。本文的在线版本(doi：10.1186/s13568-015-0115-6)包含补充材料，可供授权用户使用。
Clostridium cellulovorans is an anaerobic, cellulolytic bacterium, capable of effectively degrading and metabolizing various types of substrates, including cellulose, hemicellulose (xylan and galactomannan), and pectin. Among Clostridia, this ability to degrade and metabolize a wide range of hemicellulose and pectin substrates is a unique feature; however, the mechanisms are currently unknown. To clarify the mechanisms of hemicelluloses and pectin recognition and metabolism, we carried out a quantitative proteome analysis of C. cellulovorans cultured with these substrates. C. cellulovorans was cultured in the medium of glucose (control), xylan, galactomannan (Locus bean gum, LBG), or pectin for 36 h. Xylan and galactomannan were used to search for the common recognition mechanisms of hemicellulose, and pectin was used to search for unique recognition systems in C. cellulovorans. Using an isobaric tag method and liquid chromatograph/mass spectrometer equipped with a long monolithic silica capillary column, we identified 734 intracellular proteins from all substrates. We performed KEGG analyses and cluster analyses of the resulting proteins. In the KEGG analyses, we found common degradation mechanisms for hemicellulose and pectin. In the cluster analysis corresponding to the genome analysis, we detected substrate-specific clusters that include genes involved in substrate recognition, substrate degradation, and metabolism. Combining the results of the KEGG analyses and cluster analyses, we propose the mechanisms involved in the recognition and metabolism of hemicellulose and pectin in C. cellulovorans. The online version of this article (doi:10.1186/s13568-015-0115-6) contains supplementary material, which is available to authorized users.
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DOI：
10.1007/s10295-003-0049-x
发表时间：
2003-05-01
影响因子：
3.4
作者：
Saha, BC
通讯作者：
Saha, BC
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
DOI：
10.1073/pnas.1012175107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Nataf, Yakir;Bahari, Liat;Shoham, Yuval
通讯作者：
Shoham, Yuval
影响因子：
3.2
作者：
Peekhaus, N;Conway, T
通讯作者：
Conway, T
影响因子：
3.2
作者：
Tamaru, Yutaka;Miyake, Hideo;Ueda, Mitsuyoshi
通讯作者：
Ueda, Mitsuyoshi