A single 36-h water-only fast vastly remodels the plasma lipidome.
A single 36-h water-only fast vastly remodels the plasma lipidome.
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DOI：
10.3389/fcvm.2023.1251122
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发表时间：
2023
影响因子：
3.6
通讯作者：
Zivkovic, Angela M.
中科院分区：
文献类型：
--
作者：
Hong, Brian V.;Rhodes, Christopher H.;Agus, Joanne K.;Tang, Xinyu;Zhu, Chenghao;Zheng, Jack Jingyuan;Zivkovic, Angela M.
长期禁食的特点是限制热量摄入24小时或更长时间，作为一种改善寿命和支持健康衰老的营养方法，已引起人们的关注。 我们小组以前的研究表明，人类单次36小时的水禁食导致血浆中独特的代谢组学特征和生物活性代谢物水平的增加，这改善了巨噬细胞的功能和寿命。优美的该次要结局分析旨在研究与长期禁食相关的血浆脂质组变化，并探索与心脏代谢健康和衰老标志物的任何潜在联系。我们对20名男性和女性受试者（平均年龄，27.5 ± 4.4岁;平均BMI，24.3 ± 3.1 kg/m2）进行了一项对照初步研究，受试者处于四种代谢状态：（1）空腹过夜（基线），（2）餐后2小时进食状态（进食），（3）36小时空腹状态（空腹），（4）36小时空腹后12小时餐后2小时进食状态（进食）。 采用液相色谱和电喷雾电离质谱法分析血浆脂质组学特征。与过夜禁食和进食状态相比，36小时禁食状态下几种脂质类别，包括溶血磷脂酰胆碱（LPC）、溶血磷脂酰乙醇胺（LPE）、磷脂酰乙醇胺和三酰甘油显著减少，而游离脂肪酸、神经酰胺和鞘磷脂显著增加（P < 0.05）。经多重检验校正后，832种脂质中有245种在空腹状态下与基线相比发生了显著变化（P < 0.05）。随机森林模型显示，几种脂质物质，如LPE（18：1），LPC（18：2）和FFA（20：1）是区分禁食状态与过夜禁食和餐后状态的重要特征。我们的研究结果表明，长期禁食极大地重塑了血浆脂质组，并显着改变了几种脂质的浓度，这可能是长期禁食的敏感生物标志物。长期禁食期间脂质代谢的这些变化对心脏代谢健康和健康老龄化的管理具有重要意义，需要在更大的队列和不同的人群中进一步探索和验证。
Prolonged fasting, characterized by restricting caloric intake for 24 h or more, has garnered attention as a nutritional approach to improve lifespan and support healthy aging. Previous research from our group showed that a single bout of 36-h water-only fasting in humans resulted in a distinct metabolomic signature in plasma and increased levels of bioactive metabolites, which improved macrophage function and lifespan in C. elegans. This secondary outcome analysis aimed to investigate changes in the plasma lipidome associated with prolonged fasting and explore any potential links with markers of cardiometabolic health and aging. We conducted a controlled pilot study with 20 male and female participants (mean age, 27.5 ± 4.4 years; mean BMI, 24.3 ± 3.1 kg/m2) in four metabolic states: (1) overnight fasted (baseline), (2) 2-h postprandial fed state (fed), (3) 36-h fasted state (fasted), and (4) 2-h postprandial refed state 12 h after the 36-h fast (refed). Plasma lipidomic profiles were analyzed using liquid chromatography and electrospray ionization mass spectrometry. Several lipid classes, including lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylethanolamine, and triacylglycerol were significantly reduced in the 36-h fasted state, while free fatty acids, ceramides, and sphingomyelin were significantly increased compared to overnight fast and fed states (P < 0.05). After correction for multiple testing, 245 out of 832 lipid species were significantly altered in the fasted state compared to baseline (P < 0.05). Random forest models revealed that several lipid species, such as LPE(18:1), LPC(18:2), and FFA(20:1) were important features in discriminating the fasted state from both the overnight fasted and postprandial state. Our findings indicate that prolonged fasting vastly remodels the plasma lipidome and markedly alters the concentrations of several lipid species, which may be sensitive biomarkers of prolonged fasting. These changes in lipid metabolism during prolonged fasting have important implications for the management of cardiometabolic health and healthy aging, and warrant further exploration and validation in larger cohorts and different population groups.
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影响因子：
3.8
作者：
Dobrowolski, Piotr;Prejbisz, Aleksander;Kurylowicz, Alina;Baska, Alicja;Burchardt, Pawel;Chlebus, Krzysztof;Dzida, Grzegorz;Jankowski, Piotr;Jaroszewicz, Jerzy;Jaworski, Pawel;Kaminski, Karol;Kaplon-Cieslicka, Agnieszka;Klocek, Marek;Kukla, Michal;Mamcarz, Artur;Mastalerz-Migas, Agnieszka;Narkiewicz, Krzysztof;Ostrowska, Lucyna;Sliz, Daniel;Tarnowski, Wieslaw;Wolf, Jacek;Wylezol, Mariusz;Zdrojewski, Tomasz;Banach, Maciej;Januszewicz, Andrzej;Bogdanski, Pawel
通讯作者：
Bogdanski, Pawel
影响因子：
5.6
作者：
Mandik F;Vos M
通讯作者：
Vos M
影响因子：
3.3
作者：
Lodhi, Irfan J.;Bridges, Dave;Saltiel, Alan R.
通讯作者：
Saltiel, Alan R.
影响因子：
4.2
作者：
Kohl, Kevin D.;Amaya, James;McCue, Marshall D.
通讯作者：
McCue, Marshall D.
影响因子：
8.9
作者：
Ibrahim, M. Mohsen
通讯作者：
Ibrahim, M. Mohsen