TCS1, a Microtubule-Binding Protein, Interacts with KCBP/ZWICHEL to Regulate Trichome Cell Shape in Arabidopsis thaliana.
TCS1, a Microtubule-Binding Protein, Interacts with KCBP/ZWICHEL to Regulate Trichome Cell Shape in Arabidopsis thaliana.
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DOI：
10.1371/journal.pgen.1006266
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发表时间：
2016-10
期刊：
影响因子：
4.5
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Chen L;Peng Y;Tian J;Wang X;Kong Z;Mao T;Yuan M;Li Y
细胞形状是如何控制的是发育生物学中的一个基本问题，但决定细胞形状的遗传和分子机制在很大程度上是未知的。拟南芥的毛状体已经被用作在单细胞水平上研究细胞形状的一个很好的模型系统。在这里，我们描述了拟南芥中的毛状体细胞形状1（tcs 1）突变体与减少的毛状体分支数量。TCS 1编码含有卷曲螺旋结构域的蛋白质。药理学分析和微管动力学观察表明，TCS 1影响微管的稳定性。生化分析和活细胞成像表明，TCS 1结合微管，促进微管的组装。进一步的研究结果表明，TCS 1物理上与KCBP/ZWICHEL，微管马达参与毛分支数量的调节。遗传分析表明，kcbp/zwi在毛分支数目上对tcs 1具有上位性。因此，我们的研究结果定义了一种新的遗传和分子机制，通过这种机制，TCS 1与KCBP相互作用，通过影响微管的稳定性来调节毛状体细胞的形状。植物细胞的特殊形状不仅对它们的生物功能至关重要，而且还影响器官的整体形状。细胞形状是如何被控制的是发育生物学中的一个基本问题，而植物细胞形状调控的研究是植物生物学中一个有趣的部分。拟南芥的毛状体已经被用作在单细胞水平上研究细胞形状的一个很好的模型系统。在这项研究中，我们使用拟南芥的毛状体作为模型，以确定毛状体细胞形状1（tcs 1）的突变体与减少的毛状体分支数量。TCS 1编码微管结合蛋白，其是微管稳定性所必需的。我们进一步发现，TCS 1物理相互作用的微管电机参与毛分支数量的调节。TCS 1通过这种微管马达遗传地起作用以控制毛状体分支数量。因此，我们的研究结果为微管细胞骨架如何决定细胞形状提供了重要的见解。
How cell shape is controlled is a fundamental question in developmental biology, but the genetic and molecular mechanisms that determine cell shape are largely unknown. Arabidopsis trichomes have been used as a good model system to investigate cell shape at the single-cell level. Here we describe the trichome cell shape 1 (tcs1) mutants with the reduced trichome branch number in Arabidopsis. TCS1 encodes a coiled-coil domain-containing protein. Pharmacological analyses and observations of microtubule dynamics show that TCS1 influences the stability of microtubules. Biochemical analyses and live-cell imaging indicate that TCS1 binds to microtubules and promotes the assembly of microtubules. Further results reveal that TCS1 physically associates with KCBP/ZWICHEL, a microtubule motor involved in the regulation of trichome branch number. Genetic analyses indicate that kcbp/zwi is epistatic to tcs1 with respect to trichome branch number. Thus, our findings define a novel genetic and molecular mechanism by which TCS1 interacts with KCBP to regulate trichome cell shape by influencing the stability of microtubules. The particular shape of plant cells is not only crucial for their biological functions but also affects the overall shape of organs. How cell shape is controlled is a fundamental question in developmental biology, and the study of plant cell shape regulation is an interesting part of plant biology. Arabidopsis trichomes have been used as a good model system to investigate cell shape at the single-cell level. In this study, we use Arabidopsis trichomes as a model to identify the trichome cell shape 1 (tcs1) mutants with the reduced trichome branch number. TCS1 encodes a microtubule binding protein, which is required for the stability of microtubules. We further find that TCS1 physically interacts with a microtubule motor involved in the regulation of trichome branch number. TCS1 acts genetically with this microtubule motor to control trichome branch number. Thus, our findings provide important insights into how the microtubule cytoskeleton determines cell shape.
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