Four signature motifs define the first class of structurally related large coiled-coil proteins in plants.
Four signature motifs define the first class of structurally related large coiled-coil proteins in plants.
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DOI：
10.1186/1471-2164-3-9
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发表时间：
2002-04-09
期刊：
影响因子：
4.4
通讯作者：
Meier, I
中科院分区：
文献类型：
--
作者：
Gindullis, F;Rose, A;Patel, S;Meier, I
含有长卷曲螺旋结构域的动物和酵母蛋白参与将其他蛋白附着到细胞的大的固态成分上。长卷曲螺旋蛋白的一个亚类是核纤层蛋白，它参与将染色质附着到核膜上，并且最近与人类遗传疾病有关。与其他真核生物相比，长卷曲螺旋蛋白在植物中几乎没有被研究过。我们搜索了完整的拟南芥基因组，并鉴定了一个结构相关的长卷曲螺旋蛋白家族。通过与番茄 cDNA 的序列相似性来鉴定丝状植物蛋白 (FPP)，番茄 cDNA 编码与核膜相关蛋白 MAF1 相互作用的卷曲螺旋蛋白。 FPP 家族由四个新颖的独特序列基序和由非卷曲螺旋接头分隔的两簇长卷曲螺旋结构域定义。所有家族成员均在多种拟南芥组织中表达。在单子叶水稻中鉴定出具有相同结构特征的同源物，表明被子植物之间的保守性。除肌球蛋白外，这是植物中长卷曲螺旋蛋白家族的首次表征。 FPP 家族的番茄同源物在酵母双杂交测定中与核膜相关蛋白结合。这可能表明 FPP 家族成员在核膜生物学中发挥作用。由于完整的拟南芥基因组似乎不包含核纤层蛋白基因，因此研究其他长卷曲螺旋蛋白很有意义，这些蛋白可能在功能上取代植物界中的核纤层蛋白。
Animal and yeast proteins containing long coiled-coil domains are involved in attaching other proteins to the large, solid-state components of the cell. One subgroup of long coiled-coil proteins are the nuclear lamins, which are involved in attaching chromatin to the nuclear envelope and have recently been implicated in inherited human diseases. In contrast to other eukaryotes, long coiled-coil proteins have been barely investigated in plants. We have searched the completed Arabidopsis genome and have identified a family of structurally related long coiled-coil proteins. Filament-like plant proteins (FPP) were identified by sequence similarity to a tomato cDNA that encodes a coiled-coil protein which interacts with the nuclear envelope-associated protein, MAF1. The FPP family is defined by four novel unique sequence motifs and by two clusters of long coiled-coil domains separated by a non-coiled-coil linker. All family members are expressed in a variety of Arabidopsis tissues. A homolog sharing the structural features was identified in the monocot rice, indicating conservation among angiosperms. Except for myosins, this is the first characterization of a family of long coiled-coil proteins in plants. The tomato homolog of the FPP family binds in a yeast two-hybrid assay to a nuclear envelope-associated protein. This might suggest that FPP family members function in nuclear envelope biology. Because the full Arabidopsis genome does not appear to contain genes for lamins, it is of interest to investigate other long coiled-coil proteins, which might functionally replace lamins in the plant kingdom.
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影响因子：
9.2
作者：
Kjer-Nielsen, L;Teasdale, RD;Gleeson, PA
通讯作者：
Gleeson, PA
影响因子：
11.6
作者：
Gindullis, P;Peffer, NJ;Meier, I
通讯作者：
Meier, I
DOI：
10.1073/pnas.97.14.8122
发表时间：
2000-07-05
影响因子：
11.1
作者：
Guan, ZH;Prado, A;Raabe, T
通讯作者：
Raabe, T
影响因子：
5.6
作者：
Herrmann, H;Strelkov, SV;Aebi, U
通讯作者：
Aebi, U
影响因子：
4.3
作者：
Masuda, K;Haruyama, S;Fujino, K
通讯作者：
Fujino, K