Illegitimate Recombination in Bacteria
Illegitimate Recombination in Bacteria
复制标题
细菌中的非法重组
DOI：
10.1128/9781555818180.ch8
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发表时间：
1999
影响因子：
2.9
通讯作者：
B. Michel
中科院分区：
文献类型：
--
作者：
B. Michel
非法重组是一种普遍存在的现象，包括三类事件。在第一类中，重排通过短同源序列之间的重组发生。第二个类与特定于站点的元素相关联。最后一类包括所有重排，其中新连接的序列共享少于3bp的同源性，并且与已知的特异性位点没有同源性。已知氧化损伤可诱导重排。在大肠杆菌中有报道，
毛皮突变体，其中铁代谢调节缺陷导致氧化损伤增加。在细菌中，转录抑制串联重复序列之间的缺失10倍。相反，转录显示增加非同源序列之间的重组。通过滚环复制刺激转座子切除增加了支持体内复制滑动事件发生的一长串间接证据。大多数关于不合理重组的遗传学研究都是在E.大肠杆菌，无论是在染色体上或与噬菌体。短同源序列之间的大多数重组事件独立于RecA的作用而发生，因为所考虑的长度远低于E. coli最小有效加工片段（MEPS）。拓扑异构酶是通过DNA链的瞬时断裂和连接来修饰分子超螺旋的酶。第一个证明拓扑异构酶可能促进细菌重排的证据来自池田和合作者的工作。非法重组是真核生物中的一个主要问题，因为它是许多病理性疾病的起源。
Illegitimate recombination is a ubiquitous phenomenon and includes three types of events. In the first class, rearrangements occur by recombination between short homologous sequences. A second class is associated with site-specific elements. A last class groups all rearrangements in which the newly linked sequences share less than 3 bp of homology and have no homology with known specific sites. Oxidative lesions are known to induce rearrangements. Deletions by illegitimate recombination between short homologous sequences were reported in Escherichia coli
fur mutants, in which a defect in iron metabolism regulation results in increased oxidative damage. In bacteria, transcription inhibits deletion between tandemly repeated sequences 10-fold. In contrast, transcription was shown to increase recombination between nonhomologous sequence. The stimulation of transposon excision by rolling-circle replication adds to the long list of indirect evidence that supports the occurrence of the replication slippage events in vivo. Most of the genetic studies of illegitimate recombination were performed in E. coli, either on the chromosome or with bacteriophages. Most of the recombination events between short homologous sequences occur independently from the action of RecA, since the length considered is far below the E. coli minimum efficient processing segment (MEPS). Topoisomerases are enzymes that modify the supercoiling of molecules through transient breakage and ligation of DNA strands. The first evidence that topoisomerases may promote rearrangements in bacteria came from the work of Ikeda and collaborators. Illegitimate recombination is a major issue in eukaryotes, because it is at the origin of numerous pathological disorders.
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期刊：
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