Distinguishing splanchnic nerve and chromaffin cell stimulation in mouse adrenal slices with fast-scan cyclic voltammetry.
Distinguishing splanchnic nerve and chromaffin cell stimulation in mouse adrenal slices with fast-scan cyclic voltammetry.
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用快速扫描循环伏安法区分小鼠肾上腺切片中的内脏神经和嗜铬细胞刺激。
DOI：
10.1152/ajpcell.00332.2010
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Wightman,RMark
中科院分区：
文献类型：
--
作者：
Walsh,PaulL;Petrovic,Jelena;Wightman,RMark
电刺激是神经生物学和神经内分泌学研究电兴奋组织不可缺少的工具。在这项工作中，高强度的电刺激肾上腺片释放的儿茶酚胺的后果进行了研究，在碳纤维微电极上的快速扫描循环伏安法。当对双极钨刺激电极施加通常用于组织切片的电刺激（10 Hz，350 μA双相，2.0 ms/相脉冲宽度）时，观察到由快相和慢相组成的双相信号。发现该信号具有刺激依赖性，当使用较小（≤250 μA）和较短（1 ms/相位）的刺激时，信号的慢相消失。发现双相信号的慢相是河豚毒素和六甲双铵独立的，而使用这些药理学试剂大大减少了快相。观察到两种不同类型的钙反应，其中快相被废除灌注低钙缓冲液，而快相和慢相可以调制时，Ca 2+完全排除在使用EGTA的解决方案。灌注硝苯地平导致缓慢的儿茶酚胺释放减少到原始信号的29%，而快速相仅减少到药物前值的74%。根据这些结果，确定肾上腺髓质的高强度刺激不仅导致内脏神经去极化，而且还导致嗜铬细胞自身去极化，从而导致双相儿茶酚胺释放。
Electrical stimulation is an indispensible tool in studying electrically excitable tissues in neurobiology and neuroendocrinology. In this work, the consequences of high-intensity electrical stimulation on the release of catecholamines from adrenal gland slices were examined with fast-scan cyclic voltammetry at carbon fiber microelectrodes. A biphasic signal, consisting of a fast and slow phase, was observed when electrical stimulations typically used in tissue slices (10 Hz, 350 μA biphasic, 2.0 ms/phase pulse width) were applied to bipolar tungsten-stimulating electrodes. This signal was found to be stimulation dependent, and the slow phase of the signal was abolished when smaller (≤250 μA) and shorter (1 ms/phase) stimulations were used. The slow phase of the biphasic signal was found to be tetrodotoxin and hexamethonium independent, while the fast phase was greatly reduced using these pharmacological agents. Two different types of calcium responses were observed, where the fast phase was abolished by perfusion with a low-calcium buffer while both the fast and slow phases could be modulated when Ca2+was completely excluded from the solution using EGTA. Perfusion with nifedipine resulted in the reduction of the slow catecholamine release to 29% of the original signal, while the fast phase was only decreased to 74% of predrug values. From these results, it was determined that high-intensity stimulations of the adrenal medulla result in depolarizing not only the splanchnic nerves, but also the chromaffin cells themselves resulting in a biphasic catecholamine release.
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烟碱和毒蕈碱受体介导分离的豚鼠嗜铬细胞分泌儿茶酚胺
影响因子：
3.3
作者：
L. Role;R. Perlman
通讯作者：
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DOI：
10.2170/jjphysiol.47.449
发表时间：
1997
期刊：
The Japanese journal of physiology
影响因子：
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Toshio Iijima
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影响因子：
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作者：
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影响因子：
5.5
作者：
KIDOKORO, Y;RITCHIE, AK
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影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
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