The genome of the pear (Pyrus bretschneideri Rehd.).
The genome of the pear (Pyrus bretschneideri Rehd.).
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梨 (Pyrus bretschneideri Rehd.) 的基因组
DOI：
10.1101/gr.144311.112
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发表时间：
2013-02
期刊：
影响因子：
7
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Wu J;Wang Z;Shi Z;Zhang S;Ming R;Zhu S;Khan MA;Tao S;Korban SS;Wang H;Chen NJ;Nishio T;Xu X;Cong L;Qi K;Huang X;Wang Y;Zhao X;Wu J;Deng C;Gou C;Zhou W;Yin H;Qin G;Sha Y;Tao Y;Chen H;Yang Y;Song Y;Zhan D;Wang J;Li L;Dai M;Gu C;Wang Y;Shi D;Wang X;Zhang H;Zeng L;Zheng D;Wang C;Chen M;Wang G;Xie L;Sovero V;Sha S;Huang W;Zhang S;Zhang M;Sun J;Xu L;Li Y;Liu X;Li Q;Shen J;Wang J;Paull RE;Bennetzen JL;Wang J;Zhang S
报道了利用BAC-by-BAC和下一代测序相结合的梨（Pyrus bretschneideri）基因组草图。该高度杂合的物种的估计基因组大小为512.0 mb，覆盖面积为194x。包含2005个SNP标记的高密度遗传图谱将75.5%的序列固定在所有17条染色体上。梨基因组编码42,812个蛋白质编码基因，其中约28.5%编码多个同种异构体。鉴定出长度为271.9 Mb的重复序列，占梨基因组的53.1%。对蔷薇科的祖先进行了模拟，重建了9条祖先染色体。梨和苹果在大约540万至2150万年前分化，最近的全基因组重复（WGD）事件一定发生在它们分化之前的30-45亿年前，但在草莓分化之后。与苹果基因组序列相比，苹果和梨基因组的大小差异主要是由于存在以转座因子（te）为主的重复序列，而两个物种的基因区域相似。对梨果实的自交不亲和、木质化石细胞（梨果实的独特特征）、山梨醇代谢和挥发性化合物起关键作用的基因也已被鉴定出来。多个SFB候选基因以串联重复序列的形式出现在梨s位点区；而木质素合成相关基因家族的扩增以及HCT、C3'H和CCOMT基因家族的高表达则导致了g -木质素和s-木质素的高积累。α -亚麻酸代谢是梨果实香气形成的重要途径。
The draft genome of the pear (Pyrus bretschneideri) using a combination of BAC-by-BAC and next-generation sequencing is reported. A 512.0-Mb sequence corresponding to 97.1% of the estimated genome size of this highly heterozygous species is assembled with 194× coverage. High-density genetic maps comprising 2005 SNP markers anchored 75.5% of the sequence to all 17 chromosomes. The pear genome encodes 42,812 protein-coding genes, and of these, ∼28.5% encode multiple isoforms. Repetitive sequences of 271.9 Mb in length, accounting for 53.1% of the pear genome, are identified. Simulation of eudicots to the ancestor of Rosaceae has reconstructed nine ancestral chromosomes. Pear and apple diverged from each other ∼5.4–21.5 million years ago, and a recent whole-genome duplication (WGD) event must have occurred 30–45 MYA prior to their divergence, but following divergence from strawberry. When compared with the apple genome sequence, size differences between the apple and pear genomes are confirmed mainly due to the presence of repetitive sequences predominantly contributed by transposable elements (TEs), while genic regions are similar in both species. Genes critical for self-incompatibility, lignified stone cells (a unique feature of pear fruit), sorbitol metabolism, and volatile compounds of fruit have also been identified. Multiple candidate SFB genes appear as tandem repeats in the S-locus region of pear; while lignin synthesis-related gene family expansion and highly expressed gene families of HCT, C3′H, and CCOMT contribute to high accumulation of both G-lignin and S-lignin. Moreover, alpha-linolenic acid metabolism is a key pathway for aroma in pear fruit.
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影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
影响因子：
4.4
作者：
Chutimanitsakun Y;Nipper RW;Cuesta-Marcos A;Cistué L;Corey A;Filichkina T;Johnson EA;Hayes PM
通讯作者：
Hayes PM
DOI：
10.1073/pnas.0900906106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Fawcett, Jeffrey A.;Maere, Steven;Van de Peer, Yves
通讯作者：
Van de Peer, Yves
影响因子：
46.9
作者：
Bennetzen, Jeffrey L.;Schmutz, Jeremy;Devos, Katrien M.
通讯作者：
Devos, Katrien M.
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick