Differential gene expression at coral settlement and metamorphosis--a subtractive hybridization study.
Differential gene expression at coral settlement and metamorphosis--a subtractive hybridization study.
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DOI：
10.1371/journal.pone.0026411
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ball EE
中科院分区：
文献类型：
--
作者：
Hayward DC;Hetherington S;Behm CA;Grasso LC;Forêt S;Miller DJ;Ball EE
成功地从浮游幼虫蜕变为定居的水螅体，并在有利的条件下建立一个未来的殖民地，对珊瑚的生存至关重要。然而，与其他动物（例如青蛙和昆虫）的情况相反，人们对珊瑚变态的分子基础知之甚少。我们已经开始用以前的微阵列研究来纠正这种情况，但仍然有很多东西需要学习。在本文中，我们利用了一种不同的技术，减法杂交，来表征在这种发育过渡中差异表达的基因，并将这种方法的成功与微阵列进行比较。采用抑制减法杂交技术（SSH）对珊瑚Acropora millepora的两个转录本进行了鉴定。一个富集于沉降前较高水平表达的转录本，另一个富集于沉降后较高水平表达的转录本。虚拟北方斑点被用来证明减法杂交技术的有效性。两个库都包含编码各种功能类蛋白质的转录本，但转录调节蛋白在定居后库中更常见。大约18%的转录本与公共数据库中的其他序列没有明显的相似性。通过原位杂交进一步表征了特别感兴趣的转录本，这表明许多转录本在空间和时间上都受到调控。值得注意的是，许多转录本表现出轴向限制性表达模式，这与它们被分离出来的基因池有关。一些转录本以与钙化作用一致的模式表达。我们已经鉴定了200多个转录本，这些转录本在珊瑚的平流层幼虫和定居后的水螅体（Acropora millepora）之间存在差异表达。序列，推测的功能，并在某些情况下的时间和空间表达的报道。
A successful metamorphosis from a planktonic larva to a settled polyp, which under favorable conditions will establish a future colony, is critical for the survival of corals. However, in contrast to the situation in other animals, e.g., frogs and insects, little is known about the molecular basis of coral metamorphosis. We have begun to redress this situation with previous microarray studies, but there is still a great deal to learn. In the present paper we have utilized a different technology, subtractive hybridization, to characterize genes differentially expressed across this developmental transition and to compare the success of this method to microarray. Suppressive subtractive hybridization (SSH) was used to identify two pools of transcripts from the coral, Acropora millepora. One is enriched for transcripts expressed at higher levels at the pre-settlement stage, and the other for transcripts expressed at higher levels at the post-settlement stage. Virtual northern blots were used to demonstrate the efficacy of the subtractive hybridization technique. Both pools contain transcripts coding for proteins in various functional classes but transcriptional regulatory proteins were represented more frequently in the post-settlement pool. Approximately 18% of the transcripts showed no significant similarity to any other sequence on the public databases. Transcripts of particular interest were further characterized by in situ hybridization, which showed that many are regulated spatially as well as temporally. Notably, many transcripts exhibit axially restricted expression patterns that correlate with the pool from which they were isolated. Several transcripts are expressed in patterns consistent with a role in calcification. We have characterized over 200 transcripts that are differentially expressed between the planula larva and post-settlement polyp of the coral, Acropora millepora. Sequence, putative function, and in some cases temporal and spatial expression are reported.
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影响因子：
3.7
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
通讯作者：
Matz MV
影响因子：
4.8
作者：
Harrington, L;Fabricius, K;Negri, A
通讯作者：
Negri, A
影响因子：
4.4
作者：
Joubert C;Piquemal D;Marie B;Manchon L;Pierrat F;Zanella-Cléon I;Cochennec-Laureau N;Gueguen Y;Montagnani C
通讯作者：
Montagnani C
影响因子：
2.7
作者：
Grasso, L. C.;Negri, A. P.;Ball, E. E.
通讯作者：
Ball, E. E.
影响因子：
21.3
作者：
Jovine, L;Qi, HY;Wassarman, PM
通讯作者：
Wassarman, PM